Application of an active attachment model as a high-throughput demineralization biofilm model

• Published in: Journal of dentistry Vol. 40; no. 1; pp. 41 - 47
• Main Authors: Silva, Thiago C, Pereira, Agnes Fatima F, Exterkate, Rob A.M, Bagnato, Vanderlei S, Buzalaf, Marília A.R, Machado, Maria Aparecida de A.M, ten Cate, Jacob M, Crielaard, Wim, Deng, Dong Mei
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Ltd 01-01-2012; Elsevier; Elsevier Limited
• Subjects: Animals; Bacterial Adhesion; Biofilm model; Biofilms; Biofilms - drug effects; Biofilms - growth & development; Biological and medical sciences; Calcium - analysis; Caries-preventive agent; Cariostatic Agents - pharmacology; Cariostatic Agents - therapeutic use; Cattle; Colony Count, Microbial; Demineralization; Dental caries; Dentin - microbiology; Dentistry; Drug Evaluation, Preclinical - methods; Facial bones, jaws, teeth, parodontium: diseases, semeiology; Fundamental and applied biological sciences. Psychology; High-Throughput Screening Assays; Light; Light emitting diodes; Medical sciences; Microbial Viability - drug effects; Microbiology; Models, Biological; Mouth. Exocrine and endocrine salivary glands. Teeth. Esophagus; Non tumoral diseases; Otorhinolaryngology. Stomatology; Photochemotherapy - methods; Photodynamic therapy; Photosensitizing Agents - pharmacology; Photosensitizing Agents - therapeutic use; Streptococcus mutans; Streptococcus mutans - drug effects; Tooth Demineralization - microbiology; Tooth Demineralization - prevention & control; Vertebrates: digestive system; Biofilm model; Photodynamic therapy; Demineralization; Caries-preventive agent; Streptococcus mutans; Bovine; Stomatology; Dental disease; Biological model; Dental carie; Dentistry; Prevention; Streptococcaceae; Vertebrata; Mammalia; Animal; Biofilm; Bacteria; Micrococcales; Artiodactyla; Ungulata
• ISSN: 0300-5712; 1879-176X
• DOI: 10.1016/j.jdent.2011.09.009

Abstract Objectives To investigate the potential of an active attachment biofilm model as a high-throughput demineralization biofilm model for the evaluation of caries-preventive agents. Methods Streptococcus mutans UA159 biofilms were grown on bovine dentine discs in a high-throughput active attachment model. Biofilms were first formed in a medium with high buffer capacity for 24 h and then subjected to various photodynamic therapies (PACT) using the combination of Light Emitting Diodes (LEDs, Biotable® ) and Photogem® . Viability of the biofilms was evaluated by plate counts. To investigate treatment effects on dentine lesion formation, the treated biofilms were grown in a medium with low buffer capacity for an additional 24 h. Integrated mineral loss (IML) and lesion depth (LD) were assessed by transversal microradiography. Calcium release in the biofilm medium was measured by atomic absorption spectroscopy. Results Compared to the water treated control group, significant reduction in viability of S. mutans biofilms was observed when the combination of LEDs and Photogem® was applied. LEDs or Photogem® only did not result in biofilm viability changes. Similar outcomes were also found for dentine lesion formation. Significant lower IML and LD values were only found in the group subjected to the combined treatment of LEDs and Photogem® . There was a good correlation between the calcium release data and the IML or LD values. Conclusions The high-throughput active attachment biofilm model is applicable for evaluating novel caries-preventive agents on both biofilm and demineralization inhibition. PACT had a killing effect on 24 h S. mutans biofilms and could inhibit the demineralization process.