Differential induction of tumor necrosis factor alpha in ovine pulmonary alveolar macrophages following infection with Corynebacterium pseudotuberculosis, Pasteurella haemolytica, or lentiviruses

• Published in: Infection and Immunity Vol. 59; no. 9; pp. 3254 - 3260
• Main Authors: Ellis, J.A. (University of Wyoming, Laramie, WY), Lairmore, M.D, O'Toole, D.T, Campos, M
• Format: Journal Article
• Language: English
• Published: Washington, DC American Society for Microbiology 01-09-1991
• Subjects: AGENT PATHOGENE; Analysis of the immune response. Humoral and cellular immunity; Animals; Biological and medical sciences; Bronchoalveolar Lavage Fluid - microbiology; Cells, Cultured; CITOQUININAS; Corynebacterium - immunology; CORYNEBACTERIUM PSEUDOTUBERCULOSIS; CULTIVO DE CELULAS; CULTURE DE CELLULE; CYTOKININE; FAGOCITOS; Fundamental and applied biological sciences. Psychology; Fundamental immunology; Immunobiology; Immunoblotting; Kinetics; LENTIVIRINAE; lentivirus; Lentivirus - immunology; Lung Diseases - immunology; Lung Diseases - microbiology; Lymphokines, interleukins ( function, expression); Macrophages - microbiology; Macrophages - ultrastructure; Microspheres; ORGANISMOS PATOGENOS; OVIN; OVINOS; PASTEURELLA; Pasteurella - immunology; Pasteurella haemolytica; PHAGOCYTE; POUMON; PULMONES; Recombinant Proteins; Regulatory factors and their cellular receptors; Sheep; TOXICIDAD; TOXICITE; Tumor Necrosis Factor-alpha - biosynthesis; Induction; Secretion; Lung; Retroviridae; Respiratory system; Infection; Pasteurellaceae; Virus; Corynebacterium pseudotuberculosis; Vertebrata; Mammalia; Viral disease; Bacteriosis; Bacteria; Lentivirinae; Sheep; Actinomycetes; Corynebacteriaceae; Artiodactyla; Ungulata; Tumor necrosis factor α; Pasteurella haemolytica; Macrophage
• ISSN: 0019-9567; 1098-5522
• DOI: 10.1128/IAI.59.9.3254-3260.1991

Soluble mediators such as tumor necrosis factor alpha (TNF-alpha) may be important in the pathogenesis of many chronic pulmonary infections. We examined the ability of Corynebacterium pseudotuberculosis, Pasteurella haemolytica, and ovine lentiviruses (OvLV) to induce TNF-alpha secretion by pulmonary alveolar macrophages (PAM). Bronchoalveolar lavage cells, composed of greater than 90% PAM, were obtained from normal sheep. Bronchoalveolar lavage cells were cultured for 2, 24, 48, 72, or 168 h in endotoxin-free RPMI medium (with 10% autologous serum) or in medium containing one of the following additives: lipopolysaccharide, 1-micrometer polystyrene beads, C. pseudotuberculosis, P. haemolytica, or one of two plaque-cloned OvLV, 85/28 or 85/34. Lipopolysaccharide, C. pseudotuberculosis, and P. haemolytica induced TNF-alpha activity in PAM cultures as early as 2 h after inoculation, as assessed by a colorimetric cytotoxicity assay. This activity could be blocked by rabbit anti-recombinant bovine TnF-alpha serum. In contrast, medium alone, polystyrene beads, and productive infection by OvLV did not induce TNF-alpha activity in PAM cultures. Bacterial pathogens which infect pulmonary macrophages may elicit the secretion of TNF-alpha within the lungs and lead to the cachectic state associated with chronic pneumonia