MALDI-TOF MS improves routine identification of non-fermenting Gram negative isolates from cystic fibrosis patients

• Published in: Journal of cystic fibrosis Vol. 11; no. 1; pp. 59 - 62
• Main Authors: Fernández-Olmos, Ana, García-Castillo, María, Morosini, María-Isabel, Lamas, Adelaida, Máiz, Luis, Cantón, Rafael
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01-01-2012
• Subjects: Achromobacter - isolation & purification; Actinobacteria - isolation & purification; Burkholderia cepacia - isolation & purification; Chryseobacterium - isolation & purification; Cystic fibrosis; Cystic Fibrosis - microbiology; Gram-Negative Aerobic Bacteria - isolation & purification; Humans; MALDI-TOF MS; Non-fermenting isolates; Proteomic identification; Proteomics; Pseudomonas - isolation & purification; Pulmonary/Respiratory; Ralstonia pickettii - isolation & purification; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; MALDI-TOF MS; Cystic fibrosis; Proteomic identification; Non-fermenting isolates
• ISSN: 1569-1993; 1873-5010
• DOI: 10.1016/j.jcf.2011.09.001

Abstract Identification of non-fermenting Gram-negative bacteria (NFGNB) from cystic fibrosis (CF) patients is often limited. A collection of stored NFGNB isolates (n = 182) recovered from CF patients over a 15 year period was examined. The routinely reported identification during this period was compared with that obtained by MALDI-TOF MS. Isolates giving discrepant identification at the genus level were further analyzed by 16S rDNA sequencing. The MALDI-TOF MS system identified 94% of the isolates, including Burkholderia cepacia and Pandoraea spp. isolates, the latter previously misidentified as other NFGNB by conventional microbiological methods. Lack of identification by MALDI-TOF MS was associated with the absence of entries in the database.