Gut Microbiota Changes by an SGLT2 Inhibitor, Luseogliflozin, Alters Metabolites Compared with Those in a Low Carbohydrate Diet in db/db Mice

• Published in: Nutrients Vol. 14; no. 17; p. 3531
• Main Authors: Hata, Shinnosuke, Okamura, Takuro, Kobayashi, Ayaka, Bamba, Ryo, Miyoshi, Tomoki, Nakajima, Hanako, Kitagawa, Nobuko, Hashimoto, Yoshitaka, Majima, Saori, Senmaru, Takafumi, Okada, Hiroshi, Ushigome, Emi, Nakanishi, Naoko, Takakuwa, Hiroshi, Sasano, Ryoichi, Hamaguchi, Masahide, Fukui, Michiaki
• Format: Journal Article
• Language: English
• Published: Basel MDPI AG 27-08-2022; MDPI
• Subjects: Amino acids; Analysis; Carbohydrates; Care and treatment; Comparative analysis; Complications and side effects; Dextrose; Diabetes; Diabetes mellitus; Diet; Dosage and administration; Drug dosages; Fatty acids; Feces; Gastrointestinal system; Gene expression; Glucose; Glucose transporter; Grip strength; gut microbiota; Health aspects; Hypoglycemic agents; Instrument industry; Intestinal microflora; Liquid crystal displays; Liver; Low carbohydrate diet; luseogliflozin; Medical research; Metabolism; Metabolites; Microbiota; Microbiota (Symbiotic organisms); Muscles; Musculoskeletal system; Nutrient deficiency; Obesity; Pharmaceutical industry; Physiological aspects; Prevention; Proteins; Risk factors; Rodents; Sarcopenia; sarcopenic obesity; Skeletal muscle; sodium-glucose co-transporter-2 inhibitor; Type 2 diabetes; Japan; France; United States; United States > US
• ISSN: 2072-6643; 2072-6643
• DOI: 10.3390/nu14173531

In recent years, sarcopenic obesity has been considered central pathological factors in diabetes. This study aimed to compare the effect of luseogliflozin, a sodium-glucose co-transporter-2 inhibitor (SGLT2i), on sarcopenic obesity in comparison to that of a low-carbohydrate diet (LCD). Twenty-week-old male db/db mice were fed a normal diet (Ctrl), LCD, and normal diet with 0.01% w/w luseogliflozin (SGLT2i) for eight weeks. Skeletal muscle mass and grip strength decreased in the LCD group mice compared to those in the control group, while they increased in the SGLT2i group mice. The amino acid content in the liver, skeletal muscle, and serum were lower in the LCD group than those in the Ctrl group but increased in the SGLT2i group mice. Short-chain fatty acids in rectal feces were lower in the LCD group mice than those in the Ctrl group, whereas they were higher in the SGLT2i group mice. The abundance of Gammaproteobacteria, Enterobacteriaceae, Escherichia, Enterobacterales, and Bacteroides caccae species increased in the LCD group compared to the other two groups, whereas the abundance of Syntrophothermus lipocalidus, Syntrophomonadaceae family, Parabacteroidesdistasonis distasonis, and the genus Anaerotignum increased in the SGLT2i group. Luseogliflozin could prevent sarcopenic obesity by improving amino acid metabolism.