Herbicidins F and G, two new nucleoside antibiotics

• Published in: Journal of antibiotics Vol. 32; no. 9; p. 862
• Main Authors: Takiguchi, Y, Yoshikawa, H, Terahara, A, Torikata, A, Terao, M
• Format: Journal Article
• Language: English
• Published: Japan 1979
• Subjects: Animals; Anti-Bacterial Agents - analysis; Anti-Bacterial Agents - biosynthesis; Anti-Bacterial Agents - pharmacology; Bacteria - drug effects; Chemical Phenomena; Chemistry; Fermentation; Herbicides - analysis; Herbicides - metabolism; Mice; Purine Nucleosides - analysis; Purine Nucleosides - metabolism; Streptomyces - genetics; Streptomyces - metabolism
• ISSN: 0021-8820
• DOI: 10.7164/antibiotics.32.862

A mutant of Streptomyces saganonensis No. 4075, obtained with N-methyl-N'-nitro-N-nitrosoguanidine treatment, produced herbicidins F and G without herbicidins A and B. Isolation of the antibiotics was performed by adsorption on resinous adsorbent followed by elution with aqueous MeOH. Herbicidin F was obtained as colorless needles after extraction of the eluate using methylene dichloride. Purification of herbicidin G was completed with silica-gel chromatography to give a crystalline powder. physico-chemical characterization revealed that herbicidins F and G were new nucleoside antibiotics having an adenine moiety in their structures. There was no inhibition activity at 100 micrograms/ml of herbicidins F and G against all of bacteria and yeast tested. Herbicidin F, as well as herbicidin G, are inhibitory activity against some of fungi such as Tricophyton rubrum (MIC; 6.25 micrograms/ml), T. asteroides (6.25 micrograms/ml), T. mentagrophytes (6.25 approximately 12.5 micrograms/ml), Botrytis cinerea (12.5 micrograms/ml), Blastomyces brasiliensis (12.5 approximately 25 micrograms/ml).