Common bean breeding for resistance to anthracnose and angular leaf spot assisted by SCAR molecular markers

The objective of the present study was to assess the genetic potential of inbred carioca common bean families from five populations derived from crossings involving elite lines and a disease-resistant line (Rudá-R), and to assess the efficiency of SCAR molecular markers in selecting plants resistant...

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Published in:Crop breeding and applied biotechnology Vol. 12; no. 1; pp. 34 - 42
Main Authors: Rocha, G.S. da, Pereira, L.P.L, Carneiro, P.C.S, Paula Júnior, T.J. de, Carneiro, J.E. de S
Format: Journal Article
Language:English
Published: Vicosa Crop Breeding and Applied Biotechnology 01-03-2012
Brazilian Society of Plant Breeding
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Summary:The objective of the present study was to assess the genetic potential of inbred carioca common bean families from five populations derived from crossings involving elite lines and a disease-resistant line (Rudá-R), and to assess the efficiency of SCAR molecular markers in selecting plants resistant to anthracnose and angular leaf spot, at the time of bulk formation. Plant architecture, yield and grain type were assessed. Significant effect among families within population was observed, suggesting wide genetic variability for the characters assessed. Twenty-six superior families were selected. The families contained the greatest number of markers, identified by SCAR molecular markers in the F4 generation. Eighteen of these families were resistant to the races 65 and 453 of Colletotrichum lindemuthianum and five were resistant to the race 63.23 of Pseudocercospora griseola. Thus selection assisted by SCAR markers, in the F4 generation, was an important tool in common bean breeding. O objetivo deste trabalho foi avaliar o potencial genético de famílias endogâmicas de feijão carioca provenientes de cinco populações oriundas de cruzamentos envolvendo linhagens elites e uma fonte de resistência a doenças (Rudá-R), bem como avaliar a eficiência de marcadores moleculares SCAR na seleção de plantas resistentes a antracnose e mancha angular, por ocasião da abertura dos bulks. Foram avaliadas a arquitetura da planta, a produtividade e o aspecto de grãos. Observou-se efeito significativo entre famílias dentro de populações, indicando ampla variabilidade genética para os caracteres avaliados. Foram selecionadas 26 famílias superiores e que reuniam maior número de marcas, identificadas por marcadores SCAR na geração F4. Dessas, 18 famílias foram resistentes às raças 65 e 453 de Colletotrichum lindemuthianum e cinco resistentes à raça 63.23 de Pseudocercospora griseola. Assim, a seleção assistida por marcadores SCAR, na geração F4, mostrou ser uma ferramenta importante no melhoramento do feijoeiro.
Bibliography:F30
2012008895
H20
ISSN:1518-7853
1984-7033
1984-7033
DOI:10.1590/S1984-70332012000100005