Inhibition by acrolein of light-induced stomatal opening through inhibition of inward-rectifying potassium channels in Arabidopsis thaliana

• Published in: Bioscience, biotechnology, and biochemistry Vol. 79; no. 1; pp. 59 - 62
• Main Authors: Islam, Md. Moshiul, Ye, Wenxiu, Matsushima, Daiki, Khokon, Md. Atiqur Rahman, Munemasa, Shintaro, Nakamura, Yoshimasa, Murata, Yoshiyuki
• Format: Journal Article
• Language: English
• Published: England Taylor & Francis 02-01-2015; Oxford University Press
• Subjects: acrolein; Acrolein - metabolism; Acrolein - pharmacology; Animals; Arabidopsis - drug effects; Arabidopsis - metabolism; Arabidopsis - radiation effects; Arabidopsis Proteins - antagonists & inhibitors; Arabidopsis Proteins - genetics; Arabidopsis Proteins - metabolism; Arabidopsis thaliana; Dose-Response Relationship, Drug; Gene Expression; guard cell; inward-rectifying potassium channel; Kinetics; Light; light-induced stomatal opening; Membrane Potentials - drug effects; Oocytes - cytology; Oocytes - drug effects; Oocytes - metabolism; Patch-Clamp Techniques; Plant Cells - drug effects; Plant Cells - metabolism; Plant Cells - radiation effects; Plant Stomata - drug effects; Plant Stomata - metabolism; Plant Stomata - radiation effects; Potassium - metabolism; Potassium Channels, Inwardly Rectifying - antagonists & inhibitors; Potassium Channels, Inwardly Rectifying - genetics; Potassium Channels, Inwardly Rectifying - metabolism; Recombinant Proteins - genetics; Recombinant Proteins - metabolism; Xenopus laevis; light-induced stomatal opening; guard cell; acrolein; inward-rectifying potassium channel; Arabidopsis thaliana
• ISSN: 0916-8451; 1347-6947
• DOI: 10.1080/09168451.2014.951028

Acrolein is a reactive α,β-unsaturated aldehyde derived from lipid peroxides, which are produced in plants under a variety of stress. We investigated effects of acrolein on light-induced stomatal opening using Arabidopsis thaliana. Acrolein inhibited light-induced stomatal opening in a dose-dependent manner. Acrolein at 100 μM inhibited plasma membrane inward-rectifying potassium (K
in
) channels in guard cells. Acrolein at 100 μM inhibited K
in
channel KAT1 expressed in a heterologous system using Xenopus leaves oocytes. These results suggest that acrolein inhibits light-induced stomatal opening through inhibition of K
in
channels in guard cells.
A reactive α,β-unsaturated aldehyde, acrolein, inhibits K
in
channel currents through inhibition of KAT1, resulting in inhibition of light-induced stomatal opening.