Effect of recombinant basic fibroblast growth factor on reimplanted teeth in beagle dogs

• Published in: Oral surgery, oral medicine, oral pathology, oral radiology and endodontics Vol. 109; no. 1; pp. 142 - 148
• Main Authors: Seshima, Fumi, DDS, Ota, Mikio, DDS, PhD, Kinumatsu, Takashi, DDS, PhD, Shibukawa, Yoshihiro, DDS, PhD, Yamada, Satoru, DDS, PhD
• Format: Journal Article
• Language: English
• Published: New York, NY Mosby, Inc 2010; Elsevier
• Subjects: Animals; Biological and medical sciences; Dental Cementum - drug effects; Dental Cementum - physiology; Dentistry; Dogs; Fibroblast Growth Factor 2 - pharmacology; Growth Substances - pharmacology; Immunoenzyme Techniques; Medical sciences; Otorhinolaryngology. Stomatology; Periodontal Ligament - drug effects; Periodontal Ligament - physiology; Proliferating Cell Nuclear Antigen - analysis; Proliferating Cell Nuclear Antigen - biosynthesis; Recombinant Proteins - pharmacology; Regeneration - drug effects; Root Resorption - prevention & control; Surgery; Tooth Ankylosis - prevention & control; Tooth Replantation; Fissipedia; Carnivora; Vertebrata; Mammalia; Animal; Teeth; Basic fibroblast growth factor; Tooth; Dog
• ISSN: 1079-2104; 1528-395X
• DOI: 10.1016/j.tripleo.2009.07.055

Objectives This study assessed the effect of recombinant fibroblast growth factor (FGF-2) on reimplanted teeth. Study design Premolars were reimplanted in dogs. Roots on the left side of the mandible were treated with FGF-2, whereas roots on right side served as controls. At 2, 4, or 8 weeks after treatment, animals were humanely killed and specimens collected and processed for histopathologic examination. Results After 2 weeks, proliferating cell nuclear antigen-positive cell ratios in the experimental group were significantly greater than those in the control group ( P < .01). After 4 and 8 weeks, the experimental group showed formation of new cementum. Moreover, incidence of replacement resorption in the experimental group was significantly lower than that in the control group ( P < .01). Conclusion The results indicate that FGF-2 promotes formation of new periodontal ligament and prevents ankylosis and root resorption following reimplantation of teeth.