Isolation and characterization of human periodontal ligament (PDL) stem cells (PDLSCs) from the inflamed PDL tissue: in vitro and in vivo evaluations

• Published in: Journal of clinical periodontology Vol. 38; no. 8; pp. 721 - 731
• Main Authors: Park, Jung-Chul, Kim, Jeong-Min, Jung, Im-Hee, Kim, Jane C., Choi, Seong-Ho, Cho, Kyoo-Sung, Kim, Chang-Sung
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 01-08-2011; Blackwell
• Subjects: Adipogenesis - physiology; Adolescent; Adult; Alveolar Bone Loss - pathology; Alveolar Bone Loss - surgery; Animals; Antigens, Surface - analysis; Biological and medical sciences; Cell Culture Techniques; Cell Differentiation - physiology; Cell Movement - physiology; Cell Proliferation; Cell Separation; Cementogenesis - physiology; Chronic Periodontitis - pathology; Colony-Forming Units Assay; Dentistry; Facial bones, jaws, teeth, parodontium: diseases, semeiology; Flow Cytometry; Granulation Tissue - pathology; Humans; Immunohistochemistry; inflammation; Medical research; Medical sciences; Mesenchymal Stem Cell Transplantation; Mesenchymal Stromal Cells - cytology; Mesenchymal Stromal Cells - physiology; Mice; Middle Aged; Non tumoral diseases; Osteogenesis - physiology; Otorhinolaryngology. Stomatology; periodontal ligament; Periodontal Ligament - cytology; periodontal ligament stem cells; Periodontal Pocket - pathology; Periodontal Pocket - surgery; regeneration; Regeneration - physiology; Stem cells; Surgical Flaps - pathology; tissue engineering; Young Adult; Human; Evaluation; In vivo; Regeneration; periodontal ligament stem cells; Periodontal ligament; Tissue engineering; Stem cell; Inflammation; Dentistry; In vitro
• ISSN: 0303-6979; 1600-051X
• DOI: 10.1111/j.1600-051X.2011.01716.x

Park J‐C, Kim J‐M, Jung I‐H, Kim JC, Choi S‐H, Cho K‐S, Kim C‐S. Isolation and characterization of human periodontal ligament (PDL) stem cells (PDLSCs) from the inflamed PDL tissue: in vitro and in vivo evaluations. J Clin Periodontol 2011; 38: 721–731. doi: 10.1111/j.1600‐051X.2011.01716.x.
Objectives: Mesenchymal stem cells (MSC) could be isolated from healthy periodontal ligaments (PDL). The aims of this study were to isolate and characterize human PDL stem cells (hPDLSCs) from inflamed PDL tissue, and to evaluate their regenerative potential.
Materials and Methods: Inflamed hPDLSCs (ihPDLSCs) were isolated from the inflamed PDL tissue obtained from intra‐bony defects during flap surgery, and characterized by immunohistochemical staining, colony‐forming unit assay, fluorescence‐activated cell sorting, and mRNA expression in comparison with healthy hPDLSCs obtained from extracted teeth for orthodontic purpose. The proliferative potential and migratory potential was evaluated, and compared with healthy hPDLSCs. Regenerative potential was assessed by an in vivo ectopic transplantation model.
Results: ihPDLSCs were successfully isolated and characterized as MSCs. Both ihPDLSCs and hPDLSCs were successfully differentiated under osteogenic/cementogenic and adipogenic microenvironment. The proliferative potential did not differ between healthy hPDLSCs and ihPDLSCs, while the migratory capacity was significantly increased in ihPDLSCs (p<0.05). Both groups exhibited new cementum‐like tissue and related PDL fibre regeneration in an in vivo transplantation model.
Conclusion: ihPDLSCs could be successfully isolated from inflamed PDL tissue, and they retained the regenerative potential for cementum and related PDL tissues.