Microspore development in the anther culture of wheat (Triticum aestivum L.)

• Published in: CYTOLOGIA Vol. 55; no. 3; pp. 475 - 478
• Main Authors: Hassawi, D.S, Sears, R, Liang, G.H
• Format: Journal Article
• Language: English
• Published: Tokyo Japan Mendel Society, International Society of Cytology 1990; Japan Mendel Society; Japan Science and Technology Agency
• Subjects: Androgenesis; Anther and ovule culture, utilization for haploid production; anther culture; Biological and medical sciences; Biotechnology; callus; Eukaryotic cell cultures; Fundamental and applied biological sciences. Psychology; Methods. Procedures. Technologies; microspora; Microspore division; Plant cells and fungal cells; pollen; Triticum aestivum; Cell proliferation; Monocotyledones; Callus; Vegetals; Tissue culture; Optimization; Androgenesis; Haploidy; Gramineae; Angiospermae; Development; Spermatophyta; Pollen; Triticum aestivum
• ISSN: 0011-4545; 1348-7019
• DOI: 10.1508/cytologia.55.475

A cultivar (Chris) of hard red spring wheat (Triticum aestivum L.) and a one-step haploid generation medium were used to study microspore development and callus formation by using an anther culture technique. Cytological observations on microspore development were made 7, 10, 13 and 16 days after the anther was placed on the medium. The first mitotic division of the uninucleate microspore formed two nuclei, which were similar or different in size. Further divisions of the vegetative nucleus produced multicellular pollen grains that continued to grow and then were released from the exine. This extensive division of the vegetative nucleus could be the source of embryoid or callus generation.