Antioxidative Effect of Royal Jelly in Cisplatin-induced Testes Damage

• Published in: Urology (Ridgewood, N.J.) Vol. 74; no. 3; pp. 545 - 551
• Main Authors: Silici, Sibel, Ekmekcioglu, Oguz, Eraslan, Gokhan, Demirtas, Abdullah
• Format: Journal Article
• Language: English
• Published: New York, NY Elsevier Inc 01-09-2009; Elsevier
• Subjects: Animals; Antioxidants - therapeutic use; Biological and medical sciences; Cisplatin - administration & dosage; Cisplatin - adverse effects; Fatty Acids - therapeutic use; Insect Hormones - therapeutic use; Male; Medical sciences; Nephrology. Urinary tract diseases; Oxidative Stress; Rats; Rats, Wistar; Testicular Diseases - chemically induced; Testicular Diseases - metabolism; Testicular Diseases - prevention & control; Testis - drug effects; Testis - pathology; Urology; Antineoplastic agent; Royal jelly; Alkylating agent; Nephrology; Lesion; Antioxidant; Testicle; Male genital system; Cisplatin; Urology; Platinum II Complexes
• ISSN: 0090-4295; 1527-9995
• DOI: 10.1016/j.urology.2009.05.024

Objectives To investigate the antioxidative effect of royal jelly on cisplatin (CP)-induced spermiotoxicity using quantitative, biochemical, and histopathologic approaches. Methods CP was administered to rats at a single dose of 7 mg/kg i.p. Royal jelly was administered by gavage daily for 10 days at doses of 50 and 100 mg/kg. Traits of reproductive organs, such as sperm characteristics, testicular histologic findings, plasma testosterone levels, and testicular tissue oxidative stress status were determined. Results Royal jelly ameliorated the CP-induced reductions in weights of testes, epididymides, seminal vesicles, and prostate along with epididymal sperm concentration and motility. An increase in testes malondialdehyde concentrations ( P <.05) were detected, while significant decreases in superoxide dismutase, catalase, and glutathione-peroxidase levels were noted in CP-alone group compared with control group. The administration of royal jelly to CP-treated rats decreased the malondialdehyde level and increased superoxide dismutase, catalase, and glutathione-peroxidase activities in the samples. Conclusions The CP-induced changes in histopathologic findings of testis were partially reversed by treatment with royal jelly. The results provide further insight into the mechanisms of CP-induced sperm toxicity and confirm the antioxidant potential of royal jelly.