IR-enhanced photothermal therapeutic effect of graphene magnetite nanocomposite on human liver cancer HepG2 cell model

• Published in: International journal of nanomedicine Vol. 14; pp. 4397 - 4412
• Main Authors: Salaheldin, Taher A, Loutfy, Samah A, Ramadan, Marwa A, Youssef, Tareq, Mousa, Shaker A
• Format: Journal Article
• Language: English
• Published: New Zealand Dove Medical Press Limited 01-06-2019; Dove; Dove Medical Press
• Subjects: Actin; Apoptosis; Cancer; cytotoxicity; DNA; Electron microscopy; Genes; Genetic aspects; Graphene; graphene magnetite nanocomposite; Graphite; Health aspects; HepG2 human liver cancer; Iron oxides; Lasers; Liver cancer; Magnetite; Messenger RNA; Microscopy; Muscle proteins; Nanoparticles; Original Research; PCR; photothermal effect; Raman spectroscopy; RNA; Spectroscopy; Spinel group; Transcription (Genetics); Netherlands; Egypt; HepG2 human liver cancer; photothermal effect; graphene magnetite nanocomposite; PCR; cytotoxicity
• ISSN: 1178-2013; 1176-9114; 1178-2013
• DOI: 10.2147/IJN.S196256

Graphene magnetite nanocomposites (G/Fe
O
) exhibit light photothermal conversion upon enhancement by 808 nm IR laser excitation. We evaluated the cytotoxic and photothermal effects of G/Fe
O
on a HepG2 human liver cancer cell model.
Graphene nanosheets (rGO), magnetite nanoparticles (Fe
O
), and G/Fe
O
were prepared by chemical methods and characterized using transmission electron microscopy, Raman spectroscopy, zeta analysis, and vibrating sample magnemeter. Dark and light cytotoxicity were screened with colorimetric Sulforhodamine B cell viability assay after 24 and 48 hours. DNA fragmentation and some apoptotic genes on a transcriptional RNA level expression were performed. All prepared nanomaterials were evaluated for their photothermal effect at concentrations of 10 and 50 µg/mL. The power density incident on the cells by 300 mW 808 IR diode laser was 0.597 W/cm
.
Treatment of HepG2 with 400 µg/mL of rGO, Fe
O
, and G/Fe
O
showed alteration in cell morphology after 24 hours of cell treatment and revealed toxic effects on cellular DNA. Evaluation of the cytotoxic effects showed messenger RNA (mRNA) in
and
apoptotic genes, but no expression of mRNA of
after 24 hours of cell exposure, suggesting the involvement of an intrinsic apoptotic caspase-independent pathway. A photothermal effect was observed for G/Fe
O
after irradiation of the HepG2 cells. A marked decrease was found in cell viability when treated with 10 and 50 µg/mL G/Fe
O
from 40% to 5% after 48 hours of cell treatment.
Results indicate that G/Fe
O
nanocomposite was effective at transformation of light into heat and is a promising candidate for cancer therapy.