Effects of salinomycin and 17-AAG on proliferation of human gastric cancer cells in vitro

• Published in: Molecular medicine reports Vol. 16; no. 2; pp. 1063 - 1070
• Main Authors: Zhang, Zuwen, Zhao, Jumei, Mi, Zhikuan, Pang, Qiuxia, Wang, Aihong, Chen, Meini, Liu, Xiaobin, Wei, Xiaoli, Liu, Tao
• Format: Journal Article
• Language: English
• Published: Greece D.A. Spandidos 01-08-2017; Spandidos Publications; Spandidos Publications UK Ltd
• Subjects: 17-AAG; Apoptosis; Apoptosis - drug effects; Benzoquinones - pharmacology; Cancer cells; Cancer therapies; Cell cycle; Cell Cycle - drug effects; Cell growth; Cell Line, Tumor; Cell proliferation; Cell Proliferation - drug effects; Cell Shape - drug effects; Cell Survival - drug effects; Development and progression; Fas Ligand Protein - metabolism; fas Receptor - metabolism; Fas-L; Flow cytometry; Gastric cancer; Gram-positive bacteria; Health aspects; Humans; Immunocytochemistry; Lactams, Macrocyclic - pharmacology; Microscopes; Morphology; NF-kappa B - metabolism; NF-κB p65; NF-κB protein; Polyclonal antibodies; Protein expression; Proteins; Pyrans - pharmacology; S phase; Salinomycin; Signal Transduction - drug effects; Stomach cancer; Stomach Neoplasms - metabolism; Stomach Neoplasms - pathology; Studies; United States > US; China
• ISSN: 1791-2997; 1791-3004
• DOI: 10.3892/mmr.2017.6735

The aim of the present study was to investigate the effects and mechanisms of 17-AAG combined with salinomycin treatment on proliferation and apoptosis of the SGC-7901 gastric cancer cell line. An MTT assay was used to detect the proliferation of SGC-7901 cells. Morphological alterations of cells were observed under inverted phase-contrast and fluorescence microscopes. Cell cycle and apoptosis were assessed by flow cytometry analysis. The protein expression of nuclear factor (NF)-κB p65 and Fas-ligand (L) were evaluated by immunocytochemistry. Salinomycin with a concentration range of 1-32 µmol/l was demonstrated to inhibit growth of SGC-7901 cells effectively, affect the morphology and apoptosis rate of cells, and arrest SGC-7901 cells in S phase. Furthermore, salinomycin significantly increased the protein expression of Fas-L and decreased the protein expression of NF-κB p65. The alterations in SGC-7901 cells co-treated with salinomycin and 17-AAG were more significant compared with cells treated with one drug only. In conclusion, the individual use of salinomycin and combined use with 17-AAG may significantly inhibit SGC-7901 gastric cancer cell proliferation and induce cell apoptosis. The potential mechanisms may be associated with upregulation of Fas-L and downregulation of NF-κB. These results provide a basis for the potential use of salinomycin in gastric cancer treatment.