Inhibitory Effect of Gardenoside on Free Fatty Acid-Induced Steatosis in HepG2 Hepatocytes

• Published in: International journal of molecular sciences Vol. 16; no. 11; pp. 27749 - 27756
• Main Authors: Liang, Huiqing, Zhang, Limin, Wang, Hongguo, Tang, Jinmo, Yang, Jiaen, Wu, Chuncheng, Chen, Shaodong
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 20-11-2015; MDPI
• Subjects: Cell Survival - drug effects; Cytokines - metabolism; Fatty acids; Fatty Acids, Nonesterified - pharmacology; Fatty Acids, Nonesterified - toxicity; Fatty Liver - etiology; Fatty Liver - metabolism; Fatty Liver - pathology; free fatty acid; gardenoside; Hep G2 Cells; Hepatocytes - drug effects; Hepatocytes - metabolism; Hepatocytes - pathology; Hepatology; HepG2; Herbs; Humans; Inflammation Mediators - metabolism; inflammatory cytokines; Iridoids - pharmacology; Lipid Metabolism - drug effects; NF-kappa B - metabolism; NFκB; Phosphorylation; steatosis; HepG2; gardenoside; inflammatory cytokines; NFκB; steatosis; free fatty acid
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms161126058

Gardenoside is one of the most important effective extractions of a herb for its hepatoprotective properties. The aim of this study was to address the mechanism of Gardenoside on HepG2 cellular steatosis induced by free fatty acids (FFAs). The model of HepG2 steatosis was duplicated by oleic and palmitic acid at the proportion of 2:1 (FFAs mixture) for 24 h, then lipid toxicity was induced. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) were used to detect cell viability and Oil Red O staining method was used to judge the lipid accumulation respectively. Inflammatory cytokines TNF-α, IL-1β, IL-6 and intracellular NFκB were measured after 24 h. The steatosis was significantly decreased after Gardenoside treatment without cytotoxicity. TNF-α, IL-1β, IL-6 were modulated to HepG2 cells by treatment of Gardenoside. In the meantime, the activation of NFκB was inhibited by Gardenoside. Gardenoside has a protective effect on FFA-induced cellular steatosis in HepG2 cells which indicates that Gardenoside might be a potential therapeutic herb against NASH by suppressed supernatant inflammatory cytokine production and intracellular NFkB activity.