Genotoxicity of Superparamagnetic Iron Oxide Nanoparticles in Granulosa Cells

• Published in: International journal of molecular sciences Vol. 16; no. 11; pp. 26280 - 26290
• Main Authors: Pöttler, Marina, Staicu, Andreas, Zaloga, Jan, Unterweger, Harald, Weigel, Bianca, Schreiber, Eveline, Hofmann, Simone, Wiest, Irmi, Jeschke, Udo, Alexiou, Christoph, Janko, Christina
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 03-11-2015; MDPI
• Subjects: cancer therapy and diagnosis; Cell Line; Cell Survival - drug effects; Cells; Dextrans - metabolism; Dextrans - toxicity; DNA Damage - drug effects; Female; Ferric Compounds - metabolism; Ferric Compounds - toxicity; Genotype & phenotype; granulosa cells; Granulosa Cells - drug effects; Granulosa Cells - metabolism; Humans; Iron; Magnetic fields; Magnetite Nanoparticles - toxicity; Micronuclei, Chromosome-Defective - drug effects; Mutagenicity Tests; Nanoparticles; protein corona; reproductive health; superparamagnetic iron oxide nanoparticles; Toxicity; cancer therapy and diagnosis; granulosa cells; reproductive health; superparamagnetic iron oxide nanoparticles; protein corona
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms161125960

Nanoparticles that are aimed at targeting cancer cells, but sparing healthy tissue provide an attractive platform of implementation for hyperthermia or as carriers of chemotherapeutics. According to the literature, diverse effects of nanoparticles relating to mammalian reproductive tissue are described. To address the impact of nanoparticles on cyto- and genotoxicity concerning the reproductive system, we examined the effect of superparamagnetic iron oxide nanoparticles (SPIONs) on granulosa cells, which are very important for ovarian function and female fertility. Human granulosa cells (HLG-5) were treated with SPIONs, either coated with lauric acid (SEONLA) only, or additionally with a protein corona of bovine serum albumin (BSA; SEON(LA-BSA)), or with dextran (SEON(DEX)). Both micronuclei testing and the detection of γH2A.X revealed no genotoxic effects of SEON(LA-BSA), SEON(DEX) or SEON(LA). Thus, it was demonstrated that different coatings of SPIONs improve biocompatibility, especially in terms of genotoxicity towards cells of the reproductive system.