De novo transcriptome assembly databases for the central nervous system of the medicinal leech

• Published in: Scientific data Vol. 2; no. 1; p. 150015
• Main Authors: Hibsh, Dror, Schori, Hadas, Efroni, Sol, Shefi, Orit
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 28-04-2015; Nature Publishing Group
• Subjects: 631/114/2785; 631/1647/514/1949; 631/208/212/2019; 692/698/1688/1366; Animals; Base Sequence; BASIC BIOLOGICAL SCIENCES; Central Nervous System - physiology; Data Descriptor; Databases, Genetic; Hirudinea; Hirudo medicinalis; Hirudo medicinalis - anatomy & histology; Hirudo medicinalis - genetics; Humanities and Social Sciences; multidisciplinary; Science; Science & Technology - Other Topics; Transcriptome
• ISSN: 2052-4463; 2052-4463
• DOI: 10.1038/sdata.2015.15

The study of non-model organisms stands to benefit greatly from genetic and genomic data. For a better understanding of the molecular mechanisms driving neuronal development, and to characterize the entire leech
Hirudo medicinalis
central nervous system (CNS) transcriptome we combined Trinity for
de-novo
assembly and Illumina HiSeq2000 for RNA-Seq. We present a set of 73,493
de-novo
assembled transcripts for the leech, reconstructed from RNA collected, at a single ganglion resolution, from the CNS. This set of transcripts greatly enriches the available data for the leech. Here, we share two databases, such that each dataset allows a different type of search for candidate homologues. The first is the raw set of assembled transcripts. This set allows a sequence-based search. A comprehensive analysis of which revealed 22,604 contigs with high e-values, aligned versus the Swiss-Prot database. This analysis enabled the production of the second database, which includes correlated sequences to annotated transcript names, with the confidence of BLAST best hit.
Design Type(s)
replicate design • transcription profiling by high throughput sequencing design
Measurement Type(s)
transcription profiling assay
Technology Type(s)
RNA sequencing
Factor Type(s)
replicate analysis
Sample Characteristic(s)
Hirudo medicinalis • ganglion
Machine-accessible metadata file describing the reported data
(ISA-Tab format)