Development and characterization of microsatellite markers in Campomanesia adamantium, a native plant of the Cerrado ecoregions of South America

• Published in: Applications in plant sciences Vol. 7; no. 9; pp. e11287 - n/a
• Main Authors: Crispim, Bruno do Amaral, Déo, Thamiris Gatti, Fernandes, Juliana dos Santos, Vasconcelos, Adrielle Ayumi, Vieira, Maria do Carmo, Carnevali, Thiago de Oliveira, Bajay, Miklos Maximiliano, Zucchi, Maria Imaculada, Barufatti, Alexeia
• Format: Journal Article
• Language: English
• Published: United States John Wiley & Sons, Inc 01-09-2019; John Wiley and Sons Inc; Wiley
• Subjects: Alleles; Annealing; Biodiversity; Campomanesia sessiliflora; Cloning; cross‐amplification; Deoxyribonucleic acid; Design; DNA; Ecosystems; Genetic diversity; Genetic markers; genetic structure; guavira; microsatellite loci; Microsatellites; Myrtaceae; Native species; Plant sciences; Population genetics; Population structure; Primer Note; Primer Notes; simple sequence repeat (SSR) markers; Species; Brazil; Cerrado Biome; California; Paraguay; United States > US; microsatellite loci; guavira; Campomanesia sessiliflora; cross‐amplification; Myrtaceae; genetic structure; simple sequence repeat (SSR) markers
• ISSN: 2168-0450; 2168-0450
• DOI: 10.1002/aps3.11287

Premise
A novel set of nuclear microsatellite markers was developed and characterized for Campomanesia adamantium (Myrtaceae) and tested for cross‐amplification in the related species C. sessiliflora.
Methods and Results
Forty‐one primer pairs were designed for simple sequence repeat loci, of which 36 successfully amplified and were polymorphic. The number of alleles ranged from two to 14, with an average of 8.14 alleles per locus. Additionally, cross‐amplification was tested in C. sessiliflora; more than 55.5% of the microsatellite loci amplified, confirming the use of these microsatellite markers in a related species.
Conclusions
We developed a set of microsatellite markers that will be useful for future studies of genetic diversity and population structure of C. adamantium and a closely related species, which will aid in future conservation efforts.