Hormonal Regulations of Alkaline Phosphatase, 5'-Nucleotidase and γ-Glutamyltransferase Activities in Adult Rat Hepatocytes Cultured in Serum-Free Medium Collagen Gel

• Published in: Cell Structure and Function Vol. 18; no. 1; pp. 73 - 78
• Main Authors: Horiuti, Yoshifumi, Yano, Kazuo, Shibuya, Yuzo
• Format: Journal Article
• Language: English
• Published: Kyoto Japan Society for Cell Biology 1993
• Subjects: 5'-Nucleotidase; 5'-Nucleotidase - metabolism; Alkaline phosphatase; Alkaline Phosphatase - metabolism; Animals; Biological and medical sciences; Bucladesine - pharmacology; Calcium - analysis; Calcium - pharmacology; Cell physiology; Cells, Cultured; Collagen; Culture Media, Serum-Free - chemistry; Culture Media, Serum-Free - pharmacology; Dexamethasone - pharmacology; Dose-Response Relationship, Drug; Enzyme Activation - drug effects; Epinephrine - pharmacology; Fundamental and applied biological sciences. Psychology; gamma-Glutamyltransferase - metabolism; Gels; Glucagon - pharmacology; Growth hormone; Growth Hormone - pharmacology; Hepatocyte; Hormonal regulation; Insulin - pharmacology; Liver - cytology; Liver - enzymology; Male; Molecular and cellular biology; Pyruvates - pharmacology; Rats; Rats, Wistar; Taurocholic Acid - pharmacology; Theophylline - pharmacology; γ-Glutamyltransferase; Alkaline phosphatase; Serum free medium; 5'-Nucleotidase; Rat; Enzyme; Transferases; Rodentia; Phosphoric monoester hydrolases; Esterases; Glycoproteins; Aminoacyltransferases; γ-Glutamyltransferase; Vertebrata; Mammalia; Enzymatic activity; Hepatocyte; Collagen; Hormonal regulation; Hydrolases
• ISSN: 0386-7196; 1347-3700
• DOI: 10.1247/csf.18.73

Primary hepatocytes were cultured on collagen gel in serum-free, α-modified Eagle's minimum essential medium containing 0.1 μMinsulin, 0.1 μM dexamethasone, 10 mM pyruvate and supplements such as glucagon, epinephrine or growth hormone. The activities of alkaline phosphatase, 5' -nucleotidase and γ-glutamyltransferase were assayed in cell extracts prepared from the cultures. All three enzyme activities were induced by glucagon, epinephrine or dibutyryl cAMP.The maximally effective concentration of glucagon was 5-10 nM for both alkaline phosphatase and 5' -nucleotidase and 100 nMfor γ-glutamyltransferase. Only alkaline phosphatase activity was suppressed by growth hormone, which caused marked suppression at about 1 μU (0.25 ng)/ml. Taurocholate also induced both alkaline phosphatase and γ-glutamyltransferase activities at 1 mM.