Transformation of Cell-Derived Microparticles into Quantum-Dot-Labeled Nanovectors for Antitumor siRNA Delivery

• Published in: Angewandte Chemie International Edition Vol. 54; no. 3; pp. 1036 - 1040
• Main Authors: Chen, Gang, Zhu, Jun-Yi, Zhang, Zhi-Ling, Zhang, Wei, Ren, Jian-Gang, Wu, Min, Hong, Zheng-Yuan, Lv, Cheng, Pang, Dai-Wen, Zhao, Yi-Fang
• Format: Journal Article
• Language: English
• Published: Weinheim WILEY-VCH Verlag 12-01-2015; WILEY‐VCH Verlag; Wiley Subscription Services, Inc
• Edition: International ed. in English
• Subjects: Animals; Antineoplastic Agents - chemistry; Antineoplastic Agents - metabolism; Antineoplastic Agents - therapeutic use; Apoptosis; biogenic vesicles; Biotinylation; Cell Line, Tumor; Cell Proliferation; cell-derived microparticles; Cell-Derived Microparticles - chemistry; Drug Carriers - chemistry; Exchange; Fluoresceins - chemistry; Fluorescent Dyes - chemistry; Human Umbilical Vein Endothelial Cells; Humans; Marking; Membranes; Mice; Mice, Nude; Microparticles; Microscopy, Fluorescence; nanoparticles; Nanoparticles - chemistry; Nanostructure; Neoplasms - diagnosis; Neoplasms - drug therapy; Quantum dots; Quantum Dots - chemistry; Recognition; RNA, Small Interfering - chemistry; RNA, Small Interfering - metabolism; RNA, Small Interfering - therapeutic use; Strategy; Streptavidin - chemistry; Succinimides - chemistry; targeted delivery; Transformations; Transplantation, Heterologous; Vascular Endothelial Growth Factor A - antagonists & inhibitors; Vascular Endothelial Growth Factor A - genetics; Vascular Endothelial Growth Factor A - metabolism; quantum dots; targeted delivery; nanoparticles; cell-derived microparticles; biogenic vesicles
• ISSN: 1433-7851; 1521-3773
• DOI: 10.1002/anie.201410223

Cell‐derived microparticles (MPs) have been recently recognized as critical intercellular information conveyors. However, further understanding of their biological behavior and potential application has been hampered by the limitations of current labeling techniques. Herein, a universal donor‐cell‐assisted membrane biotinylation strategy was proposed for labeling MPs by skillfully utilizing the natural membrane phospholipid exchange of their donor cells. This innovative strategy conveniently led to specific, efficient, reproducible, and biocompatible quantum dot (QD) labeling of MPs, thereby reliably conferring valuable traceability on MPs. By further loading with small interference RNA, QD‐labeled MPs that had inherent cell‐targeting and biomolecule‐conveying ability were successfully employed for combined bioimaging and tumor‐targeted therapy. This study provides the first reliable and biofriendly strategy for transforming biogenic MPs into functionalized nanovectors.
Illuminating biogenic microparticles: Cell‐derived microparticles (MPs) are transformed to functionalized nanovectors by combining quantum dot (QD) labeling and efficient siRNA loading. This strategy not only reliably conferred excellent traceability and therapeutic potential on MPs, but also preserved their natural properties, thus facilitating the identification and further application of biogenic MPs. SA=streptavidin.