Isolation of high quality RNA from bilberry (Vaccinium myrtillus L.) fruit

• Published in: Molecular biotechnology Vol. 19; no. 2; pp. 201 - 203
• Main Authors: JAAKOLA, Laura, PIRTTILÄ, Anna Maria, HALONEN, Minna, HOHTOLA, Anja
• Format: Journal Article
• Language: English
• Published: Totowa, NJ Humana Press 01-10-2001; Springer Nature B.V
• Subjects: Ammonium; b-mercaptoethanol; Berries; Biological and medical sciences; Biotechnology; Cetrimonium Compounds - pharmacology; Diverse techniques; DNA, Complementary - metabolism; Fruits; Fundamental and applied biological sciences. Psychology; hexadecyltrimethyl ammonium bromide; Mercaptoethanol - pharmacology; Molecular and cellular biology; Molecular Biology - methods; Phenols; Pine trees; Plant tissues; Plasma Substitutes - pharmacology; polyvinylpyrrolidone; Povidone - pharmacology; Ribonucleic acid; RNA; RNA - analysis; RNA - isolation & purification; Saccharides; Vaccinium - metabolism; Vaccinium myrtillus; Vaccinium myrtillus; RNA; Small fruits; Dicotyledones; Angiospermae; Spermatophyta; Extraction; Ericaceae; Method
• ISSN: 1073-6085; 1559-0305; 1073-6085
• DOI: 10.1385/mb:19:2:201

A simple and efficient method is described for isolating high quality RNA from bilberry fruit. The procedure is based on the use of hexadecyltrimethyl ammonium bromide (CTAB), polyvinylpyrrolidone (PVP), and beta-mercaptoethanol in an extraction buffer in order to eliminate the polysaccharides and prevent the oxidation of phenolic compounds. This method is a modification of the one described for pine trees, and yields high-quality RNA suitable for cDNA based methodologies. This method is applicable for a variety of plant tissues.