lncRNA SNHG7 affects malignant tumor behaviors through downregulation of EZH2 in uveal melanoma cell lines

• Published in: Oncology letters Vol. 19; no. 2; pp. 1505 - 1515
• Main Authors: Wu, Xue, Yuan, Yiqun, Ma, Ruiqi, Xu, Binbin, Zhang, Rui
• Format: Journal Article
• Language: English
• Published: Greece Spandidos Publications 01-02-2020; Spandidos Publications UK Ltd; D.A. Spandidos
• Subjects: Analysis; Apoptosis; Biotechnology; Cancer; Cancer genetics; Carcinogens; Cell cycle; Cell growth; Fluorescein; Gene expression; Genes; Genetic aspects; Genomes; Genomics; Medical prognosis; Melanoma; Membranes; Messenger RNA; Metastasis; Oncology; Polymerase chain reaction; Prognosis; Proteins; RNA; Scientific equipment industry; Transcription (Genetics); Tumors; United States; UM; tumor suppressor; lncRNA; SNHG7; EZH2
• ISSN: 1792-1074; 1792-1082
• DOI: 10.3892/ol.2019.11240

Previous studies have demonstrated that the long non-coding RNA, small nucleolar RNA host gene 7 (SNHG7) plays an important role in several types of cancer; however, its role in the development of uveal melanoma (UM) remains unclear. The present study investigated the effect of SNHG7 on the prognosis of UM, as well as on cell proliferation, cell cycle and apoptosis of UM cell lines. Furthermore, the present study aimed to determine the molecular mechanisms underlying these effects. The association between SNHG7 and prognosis of UM was analyzed using detailed SNHG7 mRNA expression data and clinical information from The Cancer Genome Atlas database. Reverse transcription-quantitative PCR was used in order to detect the differential expression of SNHG7 in UM tissues and cell lines. Cell proliferation was detected using Cell Counting Kit-8 assays, following overexpression of SNHG7. A cell cycle assay was performed using propidium iodide/RNase staining. An apoptosis assay was performed using the Annexin-V-Fluorescein isothiocyanate apoptosis detection kit. The expression of enhancer of zeste homolog 2 (EZH2) was measured via western blotting. The results of the present study indicated that low expression of SNHG7 was associated with poor prognosis. Furthermore, increasing the expression of SNHG7 inhibited the proliferation of UM cells, suppressed cell cycle progression and promoted apoptosis. Western blot analysis results revealed that overexpression of SNHG7 downregulated EZH2 protein expression levels in UM cell lines. The results of the present study demonstrated that SNHG7 inhibited malignant transformation of UM cells by regulating EZH2 expression.