Effect of Lactobacillus plantarum expressing multifunctional glycoside hydrolases on the characteristics of alfalfa silage

• Published in: Applied microbiology and biotechnology Vol. 103; no. 19; pp. 7983 - 7995
• Main Authors: Guo, Jingui, Xie, Yixiao, Yu, Zhu, Meng, Geng, Wu, Zhe
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01-10-2019; Springer; Springer Nature B.V
• Subjects: Acids; Additives; Alfalfa; Alfalfa silage; Animal Feed - microbiology; Arabinose; Biomedical and Life Sciences; Biotechnological Products and Process Engineering; Biotechnology; Carbohydrate composition; Carbohydrates; Carbohydrates - analysis; Carboxymethylcellulose; Cellulase; Cellulose; Chemical composition; Enzymes; Fermentation; Food Analysis; Fructose; Galactose; Genes; Glycoside hydrolase; Glycoside Hydrolases - genetics; Glycoside Hydrolases - metabolism; Hemicellulose; Hydrolases; Lactobacillus plantarum; Lactobacillus plantarum - enzymology; Lactobacillus plantarum - genetics; Lactobacillus plantarum - growth & development; Lactobacillus plantarum - metabolism; Life Sciences; Lignocellulose; Medicago sativa - metabolism; Metabolic Engineering; Microbial Genetics and Genomics; Microbiology; Organic chemistry; Peptides; Plasmids; Raffinose; Recombinant Proteins - genetics; Recombinant Proteins - metabolism; Silage; Silage - microbiology; Stachyose; Temperature; Xylan; China; Glycoside hydrolases; Structural carbohydrate; Nonstructural carbohydrate composition; Alfalfa silage; Lactobacillus plantarum
• ISSN: 0175-7598; 1432-0614
• DOI: 10.1007/s00253-019-10097-6

For the first time,
Lactobacillus plantarum
strains carrying heterologous genes encoding multifunctional glycoside hydrolases were constructed and used as additives for alfalfa silage. The chemical characteristics, nonstructural carbohydrate composition, and fermentation quality of alfalfa silage were examined. The supernatant of
L. plantarum
expressing CbXyn10C and Bgxg1 (LP11AG) showed activities on xylan, Avicel, and carboxymethylcellulose (CMC), while the supernatant of the wild-type
L. plantarum
showed no activity. When LP11AG was used as silage additive, the water-soluble carbohydrate content of alfalfa silage increased by 72%, 55%, and 155% compared with control when the silage was stored at 20 °C, 30 °C, and 40 °C, respectively. With LP11AG being used as an additive for the alfalfa silage stored at 20 °C, the hemicellulose, cellulose, and acid detergent ligninin (ADL) contents decreased by 17%, 6%, and 14% compared with the control (
p
< 0.05), respectively. Compared with the corresponding original contents, the contents of glucose, arabinose, galactose, and fructose detected in silage treated with LP11AG after 45 days of ensiling increased by 55%, 1494%, 68%, and 5% , respectively, when stored at 40 °C. Raffinose and stachyose, originally present in alfalfa, disappeared after ensiling. In conclusion, our results suggest that LP11AG provides a substantial benefit as a silage additive.