Comparative transcriptomic analysis reveals the coordinated mechanisms of Populus × canadensis ‘Neva’ leaves in response to cadmium stress

• Published in: Ecotoxicology and environmental safety Vol. 216; p. 112179
• Main Authors: Li, Xiang, Mao, Xiuhong, Xu, Yujin, Li, Yan, Zhao, Nan, Yao, Junxiu, Dong, Yufeng, Tigabu, Mulualem, Zhao, Xiyang, Li, Shanwen
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier Inc 15-06-2021; Elsevier
• Subjects: Cadmium stress; Environmental Sciences related to Agriculture and Land-use; Forest Science; Gene annotation; Miljö- och naturvårdsvetenskap; Physiological analysis; Populus × canadensis ‘Neva’; Skogsvetenskap; Transcription factors; Transcriptome; Populus × canadensis ‘Neva’; Cadmium stress; Transcription factors; Physiological analysis; Gene annotation; Transcriptome
• ISSN: 0147-6513; 1090-2414; 1090-2414
• DOI: 10.1016/j.ecoenv.2021.112179

Cadmium (Cd), a heavy metal element has strong toxicity to living organisms. Excessive Cd accumulation directly affects the absorption of mineral elements, inhibits plant tissue development, and even induces mortality. Populus × canadensis ‘Neva’, the main afforestation variety planted widely in northern China, was a candidate variety for phytoremediation. However, the genes relieving Cd toxicity and increasing Cd tolerance of this species were still unclear. In this study, we employed transcriptome sequencing on two Cd–treated cuttings to identify the key genes involved in Cd stress responses of P. × canadensis ‘Neva’ induced by 0 (CK), 10 (C10), and 20 (C20) mg/L Cd(NO3)2 4H2O. We discovered a total of 2,656 (1,488 up-regulated and 1,168 down-regulated) and 2,816 DEGs (1,470 up-regulated and 1,346 down-regulated) differentially expressed genes (DEGs) between the CK vs C10 and CK vs C20, respectively. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses in response to the Cd stress indicated that many DEGs identified were involved in the catalytic activity, the oxidoreductase activity, the transferase activity, and the biosynthesis of secondary metabolites. Based on the enrichment results, potential candidate genes were identified related to the calcium ion signal transduction, transcription factors, the antioxidant defense system, and transporters and showed divergent expression patterns under the Cd stress. We also validated the reliability of transcriptome data with the real-time PCR. Our findings deeper the understanding of the molecular responsive mechanisms of P. × canadensis ‘Neva’ on Cd tolerance and further provide critical resources for phytoremediation applications.
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•Cd stress presented obvious negative effects to the phenotype traits such as number of rooting and size of leaf area.•Excessive Cd accumulation directly inhibits plant tissue development.•DEGs showed divergent expression patterns under the Cd stress.•P. × canadensis ‘Neva’ was a candidate variety in future phytoremediation applications.