Quantitative evaluation of bioaerosols in different particle size fractions in dust collected on the International Space Station (ISS)

• Published in: Applied microbiology and biotechnology Vol. 103; no. 18; pp. 7767 - 7782
• Main Authors: Haines, Sarah R., Bope, Ashleigh, Horack, John M., Meyer, Marit E., Dannemiller, Karen C.
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01-09-2019; Springer; Springer Nature B.V
• Subjects: Aerosols; Aerosols - analysis; Air Microbiology; Airborne microorganisms; Analysis; Bacteria; Bacteria - genetics; Bacteria - isolation & purification; Bioaerosols; Biomedical and Life Sciences; Biotechnology; DNA sequencing; Dust; Dust - analysis; Dust control; Environmental Biotechnology; Environmental Monitoring; Food plants; Fungi; Gravity; Humans; International Space Station; Internationality; Irritation; Life Sciences; Mechanical ventilation; Microbial Genetics and Genomics; Microbiology; Microbiomes; Microbiota; Microorganisms; Nucleotide sequencing; Particle Size; Particle size distribution; Polymerase chain reaction; Quantitative analysis; Respiratory System - microbiology; Respiratory tract; Sequence Analysis, DNA; Size distribution; Skin; Space stations; Space vehicles; Spacecraft; Species; Spores; Spores, Fungal - genetics; Spores, Fungal - isolation & purification; Statistical analysis; Statistical methods; Storage conditions; Substrates; Systems design; Vacuum; Vacuum bags; Ventilation; California; Fungi; Bacteria; Dust; Particle size; Bioaerosol; International Space Station (ISS)
• ISSN: 0175-7598; 1432-0614
• DOI: 10.1007/s00253-019-10053-4

Exposure to bioaerosols can adversely influence human health through respiratory tract, eye, and skin irritation. Bioaerosol composition is unique on the International Space Station (ISS), where the size distribution of particles in the air differs from those on Earth. This is due to the lack of gravitational settling and sources of biological particles. However, we do not understand how microbes are influenced by particle size in this environment. We analyzed two types of samples from the ISS: (1) vacuum bag debris which had been sieved into five different size fractions and (2) passively collected particles on a tape substrate with a passive aerosol sampler. Using quantitative polymerase chain reaction (qPCR), the highest concentration of fungal spores was found in the 106–150 μm-sized sieved dust particles, while the highest concentration of bacterial cells was found in the 150–250 μm-sized sieved dust particles. Illumina MiSeq DNA sequencing revealed that particle size was associated with bacterial and fungal communities and statistically significant (
p
= 0.035,
p
= 0.036 respectively). Similar fungal and bacterial species were found within the passive aerosol sample and the sieved dust samples. The most abundant fungal species identified in the aerosol and sieved samples are commonly found in food and plant material. Abundant bacterial species were most associated with the oral microbiome and human upper respiratory tract. One limitation to this study was the suboptimal storage conditions of the sieved samples prior to analysis. Overall, our results indicate that microbial exposure in space may depend on particle size. This has implications for ventilation and filtration system design for future space vehicles and habitats.