Lipid peroxidation induced by carbon tetrachloride and its inhibition by antioxidant as evaluated by an oxidative stress marker, HODE

• Published in: Toxicology and applied pharmacology Vol. 208; no. 1; pp. 87 - 97
• Main Authors: Yoshida, Yasukazu, Itoh, Nanako, Hayakawa, Mieko, Piga, Rosaria, Cynshi, Osamu, Jishage, Kou-ichi, Niki, Etsuo
• Format: Journal Article
• Language: English
• Published: San Diego, CA Elsevier Inc 01-10-2005; Elsevier
• Subjects: Alanine Transaminase - blood; alpha-Tocopherol - blood; Animals; Antioxidant; Antioxidants - administration & dosage; Antioxidants - pharmacology; Ascorbic Acid - blood; Aspartate Aminotransferases - blood; Benzofurans - antagonists & inhibitors; Benzofurans - blood; Benzofurans - pharmacology; Biological and medical sciences; Biomarkers - analysis; Biomarkers - blood; Biomarkers - metabolism; BO-653; Carbon tetrachloride; Carbon Tetrachloride - administration & dosage; Carbon Tetrachloride - toxicity; Carrier Proteins - genetics; Chromatography, High Pressure Liquid; Diet; Dinoprost - analogs & derivatives; Dinoprost - blood; Fatty Acids, Unsaturated - analysis; Fatty Acids, Unsaturated - blood; Fatty Acids, Unsaturated - metabolism; Genotype; HODE; Injections, Intraperitoneal; Isoprostanes; Lipid peroxidation; Lipid Peroxidation - drug effects; Lipid Peroxides - blood; Lipid Peroxides - urine; Liver - drug effects; Liver - metabolism; Male; Medical sciences; Mice; Mice, Inbred C57BL; Mice, Knockout; Oxidative Stress; Specific Pathogen-Free Organisms; Thiobarbituric Acid Reactive Substances - metabolism; Tocotrienols - antagonists & inhibitors; Tocotrienols - blood; Tocotrienols - pharmacology; Toxicology; Ubiquinone - analogs & derivatives; Ubiquinone - blood; Vitamin E - administration & dosage; Vitamin E - pharmacology; α-Tocopherol transfer protein knockout mice; PBS; α-Tocopherol transfer protein knockout mice; Carbon tetrachloride; Lipid peroxidation; HODE; GPT; Antioxidant; GOT; Isoprostanes; αTTP; HPODE; T; BSTFA; BO-653; 8- iso-PGF 2α; T3; Oxidative stress; Vitamin; Biological marker; Lipids; α-Tocopherol; Protein; Animal; Knockout mouse; Transfer; Inhibitor; Inhibition; Peroxidation
• ISSN: 0041-008X; 1096-0333
• DOI: 10.1016/j.taap.2005.01.015

We have recently proposed total hydroxyoctadecadienoic acid (HODE) as a biomarker for oxidative stress in vivo. The biological samples such as plasma, urine, and tissues were first reduced and then saponified to convert the oxidation products of linoleate to HODE. In the present study, this method was applied to measure the oxidative damage induced by the administration of carbon tetrachloride to mice and also to evaluate the capacity of antioxidant to inhibit the above damage. α-Tocopherol transfer protein knock out (α-TTP
−/−) mice were used to evaluate antioxidant effect in the absence of α-tocopherol. The intraperitoneal administration of carbon tetrachloride to mice induced the increase in HODE in liver and plasma, which was followed by an increase in plasma glutamic-oxaloacetic transaminase (GOT) and glutamic-pyruvic transaminase (GPT). F2-isoprostanes, another prevailing biomarker, were also increased similarly, but their concentration was approximately two to three orders of magnitude smaller than that of HODE. The lipophilic antioxidants such as γ-tocopherol, γ-tocotrienol and 2,3-dihydro-5-hydroxy-4,6-di-
tert-butyl-2,2-dipentylbenzofuran (BO-653) were effective in suppressing the formation of HODE.