Assessment of Cytogenetic Damage to Exfoliated Gingival Cells in Patients with Chronic Generalized Periodontitis

• Published in: Acta clinica Croatica (Tisak) Vol. 60; no. 2; pp. 209 - 215
• Main Authors: Tadin, Antonija, Gavic, Lidia, Roguljic, Marija, Babic, Mario, Galic, Ivan, Zeljezic, Davor
• Format: Journal Article
• Language: English
• Published: Klinicki bolnicki centar Sestre milosrdnice 01-06-2021; Sestre Milosrdnice University Hospital and Institute of Clinical Medical Research, Vinogradska cesta c. 29 Zagreb; Sestre Milosrdnice University hospital, Institute of Clinical Medical Research
• Subjects: Chromosome abnormalities; Complications and side effects; Cytotoxicity; Epithelial cells; Genetic aspects; Genotoxicity; Gingival cell; Health aspects; Micronucleus assay; Original Scientific Papers; Periodontitis; Risk factors; Croatia
• ISSN: 0353-9466; 1333-9451
• DOI: 10.20471/acc.2021.60.02.06

The aim of this cross-sectional study was to investigate the occurrence of chromosomal abnormalities through the frequency of micronuclei and other genomic damage markers in patients with chronic generalized periodontitis and without periodontal disease. Micronucleus assay was performed in exfoliated gingival epithelial cells of 35 patients with generalized chronic periodontitis and 30 control subjects with healthy periodontium. Full mouth clinical examination was performed to define periodontal condition. The mean number of cells with micronuclei observed in chronic periodontitis and control groups was 1.8 ([+ or -]1.49) and 2.0 ([+ or -]1.34), respectively. Differences between the groups were not significant (p=0.S74). Compared to control subjects, patients with chronic periodontitis showed a significant increase in the number of binucleated cells (p<0.001) and number of cells with nucleoplasmic bridges (p=0.042). Study results indicated that chronic periodontitis was not associated with higher occurrence of chromosomal damage in gingival cells compared to individuals with healthy periodontium. Key words: Periodontitis; Cytotoxicity; Genotoxicity; Gingival cell; Micronucleus assay