Detection of a mammalian-like group A rotavirus in diarrhoeic chicken

• Published in: Veterinary microbiology Vol. 94; no. 1; pp. 13 - 18
• Main Authors: Wani, S.A, Bhat, M.A, Ishaq, S.M, Ashrafi, M.A, Buchh, A.S, Haq, M
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 24-06-2003; Elsevier Science
• Subjects: Animal productions; Animals; antigens; Antigens, Viral - metabolism; Biological and medical sciences; Chicken; Chickens; diarrhea; Diarrhea - veterinary; Diarrhea - virology; Electropherotype; Electrophoresis, Polyacrylamide Gel - veterinary; enzyme-linked immunosorbent assay; Enzyme-Linked Immunosorbent Assay - veterinary; Epidemiology; feces; Feces - virology; Fundamental and applied biological sciences. Psychology; Group A rotavirus; India; mammals; Microbiology; polyacrylamide gel electrophoresis; Poultry Diseases - virology; RNA; RNA, Viral - chemistry; RNA, Viral - genetics; Rotavirus; Rotavirus - classification; Rotavirus - isolation & purification; Rotavirus Infections - veterinary; Rotavirus Infections - virology; Terrestrial animal productions; Vertebrates; Virology; India; Chicken; Group A rotavirus; Electropherotype; Farming animal; Rotavirus; Reoviridae; Infection; Virus; Vertebrata; Mammalia; Viral disease; Molecular epidemiology; Aves; Veterinary; Detection
• ISSN: 0378-1135; 1873-2542
• DOI: 10.1016/S0378-1135(03)00079-8

The present investigation describes detection of a mammalian-like electropherogroup A rotavirus in chicken with diarrhoea. This also records the first detection of a rotavirus in an avian species from India. During the investigation 75 diarrhoeic faecal samples collected from adult chicken were screened for the presence of group A rotavirus antigen by sandwich ELISA. All three samples positive for rotavirus antigen revealed 11 bands of RNA in polyacrylamide gel electrophoresis (PAGE). In contrast to avian group A rotavirus, segment 5 was found to migrate closer to 6 as is the case with mammalian group A rotaviruses. Segments 7, 8 and 9 were found to migrate as a tight triplet, which is characteristic of group A rotavirus.