Structural Identification of Antibacterial Lipids from Amazonian Palm Tree Endophytes through the Molecular Network Approach

• Published in: International journal of molecular sciences Vol. 20; no. 8; p. 2006
• Main Authors: Barthélemy, Morgane, Elie, Nicolas, Pellissier, Léonie, Wolfender, Jean-Luc, Stien, Didier, Touboul, David, Eparvier, Véronique
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 24-04-2019; MDPI
• Subjects: antibacterial; Antibacterial activity; Antimicrobial agents; Astrocaryum sciophilum; Bacteria; Biochemistry, Molecular Biology; Chemical Sciences; Cytotoxicity; Endophytes; Fatty acids; Fungi; Fusarium; Leaves; Life Sciences; Lipids; Mass spectrometry; Medicinal Chemistry; Metabolites; Microorganisms; molecular networking; Pentapeptides; Peptides; Scientific imaging; Secondary metabolites; Staphylococcus infections; antibacterial; Astrocaryum sciophilum; fatty acids; molecular networking; cytotoxicity; endophytes
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms20082006

A library of 197 endophytic fungi and bacteria isolated from the Amazonian palm tree
was extracted and screened for antibacterial activity against methicillin-resistant
(MRSA). Four out of five antibacterial ethyl acetate extracts were also cytotoxic for the MRC-5 cells line. Liquid chromatography coupled to tandem mass spectrometry (UPHLC-HRMS/MS) analyses combined with molecular networking data processing were carried out to allow the identification of depsipeptides and cyclopeptides responsible for the cytotoxicity in the dataset. Specific ion clusters from the active
sp. extract were also highlighted using an MRSA activity filter. A chemical study of
sp. was conducted leading to the structural characterization of eight fatty acid exhibiting antimicrobial activity against MRSA in the tens of µg/mL range.