Positioning of centrioles is a conserved readout of Frizzled planar cell polarity signalling

Planar cell polarity (PCP) signalling is a well-conserved developmental pathway regulating cellular orientation during development. An evolutionarily conserved pathway readout is not established and, moreover, it is thought that PCP mediated cellular responses are tissue-specific. A key PCP function...

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Bibliographic Details
Published in:	Nature communications Vol. 7; no. 1; p. 11135
Main Authors:	Carvajal-Gonzalez, Jose Maria, Roman, Angel-Carlos, Mlodzik, Marek
Format:	Journal Article
Language:	English
Published:	London Nature Publishing Group UK 29-03-2016 Nature Publishing Group Nature Portfolio
Subjects:	14 14/32 14/63 631/136 631/80/85 631/80/86 82/1 82/51 96 96/106 96/44 Animals Cell Polarity Centrioles - metabolism Cytoskeleton - metabolism Drosophila melanogaster Drosophila Proteins - metabolism Frizzled Receptors - metabolism Humanities and Social Sciences multidisciplinary Pupa - cytology Science Science (multidisciplinary) Signal Transduction Wings, Animal - cytology
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Summary:	Planar cell polarity (PCP) signalling is a well-conserved developmental pathway regulating cellular orientation during development. An evolutionarily conserved pathway readout is not established and, moreover, it is thought that PCP mediated cellular responses are tissue-specific. A key PCP function in vertebrates is to regulate coordinated centriole/cilia positioning, a function that has not been associated with PCP in Drosophila . Here we report instructive input of Frizzled-PCP (Fz/PCP) signalling into polarized centriole positioning in Drosophila wings. We show that centrioles are polarized in pupal wing cells as a readout of PCP signalling, with both gain and loss-of-function Fz/PCP signalling affecting centriole polarization. Importantly, loss or gain of centrioles does not affect Fz/PCP establishment, implicating centriolar positioning as a conserved PCP-readout, likely downstream of PCP-regulated actin polymerization. Together with vertebrate data, these results suggest a unifying model of centriole/cilia positioning as a common downstream effect of PCP signalling from flies to mammals. Planar cell polarity (PCP) contributes to cellular orientation during development but how this is regulated in Drosophila is unclear. Here, the authors identify Frizzled-PCP signalling as regulating polarised centriole positioning in the wing disc.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Present address: Departamento de Bioquímica y Biología Molecular, Facultad de Ciencias, Universidad de Extremadura, Badajoz 06071, Spain.
ISSN:	2041-1723 2041-1723
DOI:	10.1038/ncomms11135