Dissolved organic matter released by two marine heterotrophic bacterial strains and its bioavailability for natural prokaryotic communities

• Published in: Environmental microbiology Vol. 23; no. 3; pp. 1363 - 1378
• Main Authors: Ortega‐Retuerta, Eva, Devresse, Quentin, Caparros, Jocelyne, Marie, Barbara, Crispi, Olivier, Catala, Philippe, Joux, Fabien, Obernosterer, Ingrid
• Format: Journal Article
• Language: English
• Published: Hoboken, USA John Wiley & Sons, Inc 01-03-2021; Wiley Subscription Services, Inc; Society for Applied Microbiology and Wiley-Blackwell
• Subjects: Alkaline phosphatase; Aminopeptidase; Bacteria; Bioavailability; Carbon; Carbon sources; Dissolved organic matter; Environmental Sciences; Glucose; Life Sciences; Metabolism; Microbiological strains; Phosphatase; Prokaryotes; Strains (organisms)
• ISSN: 1462-2912; 1462-2920
• DOI: 10.1111/1462-2920.15306

Summary
Marine heterotrophic prokaryotes (HP) play a key role in organic matter processing in the ocean; however, the view of HP as dissolved organic matter (DOM) sources remains underexplored. In this study, we quantified and optically characterized the DOM produced by two single marine bacterial strains. We then tested the availability of these DOM sources to in situ Mediterranean Sea HP communities. Two bacterial strains were used: Photobacterium angustum (a copiotrophic gammaproteobacterium) and Sphingopyxis alaskensis (an oligotrophic alphaproteobacterium). When cultivated on glucose as the sole carbon source, the two strains released from 7% to 23% of initial glucose as bacterial derived DOM (B‐DOM), the quality of which (as enrichment in humic or protein‐like substances) differed between strains. B‐DOM induced significant growth and carbon consumption of natural HP communities, suggesting that it was partly labile. However, B‐DOM consistently promoted lower prokaryotic growth efficiencies than in situ DOM. In addition, B‐DOM changed HP exoenzymatic activities, enhancing aminopeptidase activity when degrading P. angustum DOM, and alkaline phosphatase activity when using S. alaskensis DOM, and promoted differences in HP diversity and composition. DOM produced by HP affects in situ prokaryotic metabolism and diversity, thus changing the pathways for DOM cycling (e.g. respiration over biomass production) in the ocean.