Effect of glucans from Caripia montagnei mushroom on TNBS-induced colitis

• Published in: International journal of molecular sciences Vol. 15; no. 2; pp. 2368 - 2385
• Main Authors: Nascimento Santos, Marilia da S, de M Magalhães, Joedyson Emmanuel, Castro, Luiza Sheyla Evenni P Will, de Sousa Pinheiro, Thuane, Sabry, Diego Araujo, Nobre, Leonardo Thiago Duarte B, Lima, João Paulo Matos Santos, Baseia, Iuri Goulart, Leite, Edda Lisboa
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 10-02-2014; Molecular Diversity Preservation International (MDPI)
• Subjects: Agaricales - chemistry; Alkaline Phosphatase - metabolism; Animals; anti-inflammatory; Caripia montagnei; Catalase - metabolism; colitis; Colitis - chemically induced; Colitis - drug therapy; Colitis - metabolism; Colitis - pathology; Cytokines - biosynthesis; Disease Models, Animal; Enzyme Activation; Extraction processes; Glucans - administration & dosage; Glucans - chemistry; Glucans - pharmacology; Intestinal Mucosa - metabolism; Intestinal Mucosa - pathology; Male; mushroom; Mushrooms; Nitric Oxide; Nuclear Magnetic Resonance, Biomolecular; Peroxidase - metabolism; polysaccharides; Rats; Spectroscopy, Fourier Transform Infrared; Trinitrobenzenesulfonic Acid - adverse effects
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms15022368

In this study, we evaluated the effect of different doses of polysaccharides extracted from Caripia montagnei mushroom at different intervals of treatment on colonic injury in the model of colitis induced by 2,4,6-trinitrobenzene sulfonic acid (TNBS). The FT-IR analysis and NMR showed that the polysaccharides from this species of mushroom are composed of α- and β-glucans. The colonic damage was evaluated by macroscopic, histological, biochemical and immunologic analyses. The results showed the reduction of colonic lesions in all groups treated with the glucans. Such glucans significantly reduced the levels of IL-6 (50 and 75 mg/kg, p < 0.05), a major inflammatory cytokine. Biochemical analyses showed that the glucans from C. montagnei acted on reducing levels of alkaline phosphatase (75 mg/kg, p < 0.01) and myeloperoxidase (p < 0.001), a result confirmed by the reduction of cellular infiltration observed microscopically. The increase of catalase activity possibly indicates a protective effect of these glucans on colonic tissue, confirming their anti-inflammatory potential.