Effect of Elderberry (Sambucus nigra L.) Extract Supplementation in STZ-Induced Diabetic Rats Fed with a High-Fat Diet

• Published in: International journal of molecular sciences Vol. 18; no. 1; p. 13
• Main Authors: Salvador, Ângelo C, Król, Ewelina, Lemos, Virgínia C, Santos, Sónia A O, Bento, Fernanda P M S, Costa, Carina P, Almeida, Adelaide, Szczepankiewicz, Dawid, Kulczyński, Bartosz, Krejpcio, Zbigniew, Silvestre, Armando J D, Rocha, Sílvia M
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 01-01-2017; MDPI
• Subjects: Adjuvants; Animal models; Animals; Bioactive compounds; Biocompatibility; Bioluminescence; Blood Glucose - metabolism; Body weight; Diabetes; Diabetes mellitus; Diabetes Mellitus, Experimental - drug therapy; Diabetes Mellitus, Experimental - etiology; diabetic rats; Diet, High-Fat - adverse effects; Dietary Supplements; Elderberries; elderberry extracts supplementation; Evaluation; Formulations; Glucose metabolism; High fat diet; Homeostasis; Hyperglycemia; Hypoglycemic Agents - administration & dosage; Hypoglycemic Agents - chemistry; Hypoglycemic Agents - therapeutic use; Insulin; Insulin Resistance; Insulin secretion; Lipids; Lipophilic; lipophilic extract; Lipophilicity; Male; Plant Extracts - administration & dosage; Plant Extracts - chemistry; Plant Extracts - therapeutic use; polar extract; Rats; Rats, Wistar; Rodents; Sambucus; Sambucus nigra; Sambucus nigra - chemistry; Sambucus nigra L; Streptozocin; Streptozocin - toxicity; Toxicity; Trace elements; Sambucus nigra L; diabetic rats; polar extract; elderberry extracts supplementation; high-fat diet; lipophilic extract
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms18010013

Elderberry (
L.) lipophilic and polar extract dietary supplementation effects were evaluated according to diabetes management indices, using an in vivo model. A research pipeline was constructed, that ranged from extract preparation, partial chemical characterization and toxicity evaluation, to examining the elderberry extract dietary supplementation effects on biofluid and tissues. Extracts toxicity was screened using an
bioluminescence model. A concentration of up to 60 mg/L was selected, and rat doses for oral supplementation were computed applying the interspecies correlation between
and rats. Wistar type 2 diabetic rats, induced by streptozotocin (STZ), were fed a high-fat diet and supplemented for 4 weeks at doses of 190 and 350 mg/kg body weight/day of lipophilic and polar extract, respectively. As far as we know, lipophilic elderberry extract supplementation was assessed for the first time, while polar extract was administrated at higher doses and for a shorter period compared to previous studies, aiming to evaluate subacute supplementation effects. The polar extract modulated glucose metabolism by correcting hyperglycemia, while the lipophilic extract lowered insulin secretion. Both extracts lowered insulin resistance, without remarkable alterations to hematological indices, sera lipids and sera and tissular trace element homeostasis. In conclusion, elderberries are a potential source of bioactive compounds for formulations to be used as co-adjuvants in diabetes management.