Dimethyl sulfoxide perfusion in caprine ovarian tissue and its relationship with follicular viability after cryopreservation

Ovarian cortical fragments (3 × 3 × 1 mm) were exposed to dimethyl sulfoxide (DMSO) in different concentrations for further analysis of cryoprotectant perfusion by applying high-performance liquid chromatography (HPLC) and conventional cryopreservation. This simple perfusion test can predict the eff...

Full description

Saved in:

Bibliographic Details
Published in:	Fertility and sterility Vol. 91; no. 4; pp. 1513 - 1515
Main Authors:	Luz, Valesca B., D.V.M, Santos, Regiane R., Ph.D, Pinto, Leonardo C., D.V.M, Soares, Alison A.X., D.V.M, Celestino, Juliana J.H., M.S, Mafezoli, Jair, Ph.D, Campello, Cláudio C., Ph.D, Figueiredo, José R., Ph.D, Rodrigues, Ana P.R., Ph.D
Format:	Journal Article
Language:	English
Published:	New York, NY Elsevier Inc 01-04-2009 Elsevier
Subjects:	Animals Biological and medical sciences Bones, joints and connective tissue. Antiinflammatory agents Chromatography, High Pressure Liquid - methods cryopreservation Cryopreservation - methods Cryoprotective Agents - administration & dosage Cryoprotective Agents - pharmacology Dimethyl Sulfoxide - administration & dosage Dimethyl Sulfoxide - pharmacology DMSO Dose-Response Relationship, Drug Female Goats Gynecology. Andrology. Obstetrics HPLC Internal Medicine Medical sciences Models, Animal Obstetrics and Gynecology Ovarian Follicle - drug effects Ovarian Follicle - physiology Ovary - drug effects Ovary - physiology Perfusion - methods Pharmacology. Drug treatments Tissue Survival - drug effects Tissue Survival - physiology DMSO cryopreservation HPLC Prostaglandin-endoperoxide synthase Enzyme Gynecology Caprine Enzyme inhibitor HPLC chromatography Obstetrics Non steroidal antiinflammatory agent Ovary Tissue Vertebrata Mammalia Cryopreservation Perfusion Dimethyl sulfoxide Artiodactyla Oxidoreductases Viability Ungulata
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	Ovarian cortical fragments (3 × 3 × 1 mm) were exposed to dimethyl sulfoxide (DMSO) in different concentrations for further analysis of cryoprotectant perfusion by applying high-performance liquid chromatography (HPLC) and conventional cryopreservation. This simple perfusion test can predict the efficiency of the cryopreservation procedure.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0015-0282 1556-5653
DOI:	10.1016/j.fertnstert.2008.07.1778