Differential expression of genes in olive leaves and buds of ON- versus OFF-crop trees

• Published in: Scientific reports Vol. 10; no. 1; p. 15762
• Main Authors: Dastkar, Ebrahim, Soleimani, Ali, Jafary, Hossein, de Dios Alche, Juan, Bahari, Abbas, Zeinalabedini, Mehrshad, Salami, Seyed Alireza
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 25-09-2020; Nature Publishing Group
• Subjects: 631/61; 631/61/447; Abscisic acid; Buds; Carbohydrates; Chlorogenic acid; Cloning; Cytokinins; Economic importance; Flowers - genetics; Flowers - growth & development; Fruit trees; Gene Expression Profiling; Genes; Humanities and Social Sciences; Leaves; Metabolic pathways; Metabolism; Molecular modelling; multidisciplinary; Next-generation sequencing; Olea - genetics; Olea - growth & development; Olea - metabolism; Photorespiration; Plant Leaves - genetics; Polyphenol oxidase; RNA-Seq; Science; Science (multidisciplinary); Transcriptomes; Trees; Trees - genetics; Trees - growth & development
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-020-72895-7

Alternate bearing (AB) refers to the tendency of trees to have an irregular crop load from 1 year (ON) to the next year (OFF). Despite its economic importance, it is not fully understood how gene networks and their related metabolic pathways may influence the irregular bearing in olive trees. To unravel molecular mechanisms of this phenomenon in olive (cv. Conservalia), the whole transcriptome of leaves and buds from ON and OFF-trees was sequenced using Illumina next generation sequencing approach. The results indicated that expressed transcripts were involved in metabolism of carbohydrates, polyamins, phytohormones and polyphenol oxidase (POD) related to antioxidant system. Expression of POD was increased in leaf samples of ON- versus OFF-trees. The expression pattern of the greater number of genes was changed more in buds than in leaves. Up-regulation of gene homologues to the majority of enzymes that were involved in photorespiration metabolism pathway in buds of ON-trees was remarkable that may support the hypotheses of an increase in photorespiratory metabolism in these samples. The results indicated changes in expression pattern of homologous to those taking part of abscisic acid and cytokinin synthesis which are connected to photorespiration. Our data did not confirm expression of homologue (s) to those of chlorogenic acid metabolism, which has been addressed earlier that have a probable role in biennial bearing in olive. Current findings provide new candidate genes for further functional analysis, gene cloning and exploring of molecular basses of AB in olive.