Evaluation of monoclonal antibodies with specificity of human IgA, IgA subclasses and allotypes and secretory component results of an IUIS/WHO collaborative study

• Published in: Journal of immunological methods Vol. 193; no. 2; pp. 103 - 148
• Main Authors: Mestecky, J., Hamilton, R.G., Magnusson, C.G.M., Jefferis, R., Vaerman, J.P., Goodall, M., de Lange, G.G., Moro, I., Aucouturier, P., Radl, J., Cambiaso, C., Silvain, C., Preud'homme, J.L., Kusama, K., Carlone, G.M., Biewenga, J., Kobayashi, K., Skvaril, F., Reimer, C.B.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 21-06-1996; Elsevier
• Subjects: Am allotype; Animals; Antibodies, immunoglobulins; Antibodies, Monoclonal - chemistry; Antibodies, Monoclonal - classification; Antibody Specificity; Binding Sites, Antibody; Biological and medical sciences; Epitopes - chemistry; Epitopes - immunology; Fundamental and applied biological sciences. Psychology; Fundamental immunology; Human; Humans; IgA; IgA subclass; Immunoglobulin A - chemistry; Immunoglobulin A - classification; Immunoglobulin A - immunology; Immunoglobulin A, Secretory - immunology; Immunoglobulin Allotypes - chemistry; Immunoglobulin Allotypes - immunology; Immunologic Techniques - standards; Medicin och hälsovetenskap; Mice; Molecular immunology; Monoclonal antibodies; Monoclonal antibody; Reference Standards; Secretory Component - chemistry; Secretory Component - immunology; Specificity; Human; PBS; IgA; sIgA; HRP; non-IgA2m; Am allotype; Monoclonal antibody; C H; IgA PAN; AP; SC; BSA; Specificity; McAb; NP-BSA; IgA subclass; Fc; SCf,b; Ig; Secretory component; Allotype; Antigenicity; Isotype; IgA1; IgA2
• ISSN: 0022-1759; 1872-7905
• DOI: 10.1016/0022-1759(95)00289-8

51 monoclonal antibodies (McAb) with putative specificity for human IgA, the IgA subclasses, Am allotypes or secretory component (SC) were evaluated for immunoreactivity and specificity by nine laboratories employing immunodiffusion, agglutination, immunohistological assays, immunoblotting and direct binding and competitive inhibition enzyme immunoassays. McAbs specific for IgA PAN (
n = 24), IgA1 (
n = 7), IgA2 (
n = 3), IgA2m(2) (
n = 2), non-IgA2m(2) (
n = 4) and SC or secretory IgA (
n = 5) were identified that were immunoreactive and specific in the assays employed. The McAbs identified as IgA- or SC-reactive were shown to be non-reactive to human IgG, IgM, IgD, IgE, κ and λ by direct binding and competitive inhibition immunoassays. Interestingly, no McAbs with restricted specificity for IgA2m(1) were identified. Some McAbs displayed higher affinity and/or better performance in one or several of the assay groups. The IgA- and SC-specific McAbs identified in this international collaborative study have potential as immunochemical reference reagents to identify and quantitate monomeric and polymeric IgA in human serum and secretions.