Acute Restraint Stress in Zebrafish: Behavioral Parameters and Purinergic Signaling

• Published in: Neurochemical research Vol. 36; no. 10; pp. 1876 - 1886
• Main Authors: Piato, Angelo L., Rosemberg, Denis B., Capiotti, Katiucia M., Siebel, Anna M., Herrmann, Ana P., Ghisleni, Gabriele, Vianna, Monica R., Bogo, Maurício R., Lara, Diogo R., Bonan, Carla D.
• Format: Journal Article
• Language: English
• Published: Boston Springer US 01-10-2011; Springer Nature B.V
• Subjects: Adenosine Deaminase - genetics; Adenosine Deaminase - metabolism; Adenosine Triphosphatases - genetics; Adenosine Triphosphatases - metabolism; Adenosine Triphosphate - metabolism; Animals; Behavior, Animal - physiology; Biochemistry; Biomedical and Life Sciences; Biomedicine; Cell Biology; Danio rerio; Freshwater; Male; Memory - physiology; Motor Activity - physiology; Neurochemistry; Neurology; Neurosciences; Original Paper; Purines - metabolism; Restraint, Physical; Signal Transduction - physiology; Stress, Psychological; Zebrafish - physiology; Zebrafish Proteins - genetics; Zebrafish Proteins - metabolism; Acute restraint stress (ARS); Zebrafish; Nucleotide hydrolysis; Adenosine; Behavior; Memory
• ISSN: 0364-3190; 1573-6903
• DOI: 10.1007/s11064-011-0509-z

Despite the extensive knowledge about the effects of acute restraint stress (ARS) in rodents, zebrafish research is still elementary in this field, and the consequences of stress on purinergic system are unclear. Therefore, we evaluated the effects of ARS on behavior, biochemical, and molecular parameters in zebrafish brain. Animals were submitted to a 90 min ARS protocol and tested for anxiety levels, exploratory behavior, and memory performance. Furthermore, we analyzed ectonucleotidase and adenosine deaminase activities and their gene expression profile, as well as transcription of adenosine receptors. ARS increased anxiety, but did not impair locomotion or cognition. ARS significantly increased ATP hydrolysis, decreased cytosolic ADA activity, and changed the
entpd
and
adora
gene expression. In conclusion, ARS disturbed zebrafish behavior, and we hypothesize that the augmentation in adenosine-mediated signaling may be a strategy to reestablish homeostasis and normal behavior after a stressful event.