Analysis of the systemic colonization of cucumber plants by Cucumber green mottle mosaic virus

• Published in: Journal of general virology Vol. 85; no. 3; pp. 749 - 759
• Main Authors: Moreno, I.M, Thompson, J.R, Garcia-Arenal, F
• Format: Journal Article
• Language: English
• Published: Reading Soc General Microbiol 01-03-2004; Society for General Microbiology
• Subjects: Biological and medical sciences; Cotyledon - virology; Cucumber green mottle mosaic virus; Cucumis sativus - virology; Cucumovirus - isolation & purification; Cucumovirus - pathogenicity; Cucumovirus - physiology; Cucumovirus - ultrastructure; Fundamental and applied biological sciences. Psychology; Microbiology; Microscopy, Immunoelectron; Miscellaneous; Plant Diseases - virology; Plant Leaves - virology; Virology; Virus Replication; Microbiology; Cucurbitaceae; Tobamovirus; Systemic; Cucumis sativus; Virology; Virus; Dicotyledones; Angiospermae; Cucumber green mottle mosaic virus; Spermatophyta; Disseminated; Colonization
• ISSN: 0022-1317; 1465-2099
• DOI: 10.1099/vir.0.19540-0

Departamento de Biotecnología, ETSI Agrónomos, Universidad Politécnica de Madrid, 28040 Madrid, Spain
Correspondence F. García-Arenal fga{at}bit.etsia.upm.es
Systemic movement of Cucumber green mottle mosaic virus (CGMMV) in cucumber plants was shown to be from photoassimilate source to sink, thus indicating phloem transport. Nevertheless, CGMMV was not detected by immunocytochemical procedures in the intermediary cell–sieve element complex in inoculated cotyledons, where photoassimilate loading occurs. In stem internodes, CGMMV was first localized in the companion cells of the external phloem and subsequently in all tissues except the medulla, therefore suggesting leakage of the virus from, and reloading into, the transport phloem during systemic movement. In systemically infected sink leaves, CGMMV was simultaneously detected in the xylem and phloem. Interestingly, CGMMV accumulated to high levels in the differentiating tracheids of young leaves implying that the xylem could be involved in the systemic movement of CGMMV. This possibility was tested using plants in which cell death was induced in a portion of the stem by steam treatment. At 24 °C, steam treatment effectively prevented the systemic movement of CGMMV, even though viral RNA was detected in washes of the xylem above the steamed internode suggesting that xylem circulation occurred. At 29 °C, CGMMV systemically infected steam-treated cucumber plants, indicating that CGMMV can move systemically via the xylem. Xylem transport of CGMMV was, however, less efficient than phloem transport in terms of the time required for systemic infection and the percentage of plants infected.
Present address: Department of Plant Pathology, Cornell University, Ithaca, NY 14853, USA.