Identification of wheat chromosomal regions containing expressed resistance genes

• Published in: Genetics (Austin) Vol. 166; no. 1; pp. 461 - 481
• Main Authors: Dilbirligi, M, Erayman, M, Sandhu, D, Sidhu, D, Gill, K.S
• Format: Journal Article
• Language: English
• Published: United States Genetics Soc America 2004; Genetics Society of America
• Subjects: Base Sequence; Chromosomes; Chromosomes, Plant - genetics; DNA, Plant - genetics; Gene expression; Genes; Genes, Plant; Genetics; Phenotype; Physical Chromosome Mapping; Plant Diseases - genetics; Plant Diseases - microbiology; Plant Diseases - parasitology; RNA, Plant - genetics; RNA, Plant - isolation & purification; Triticum - genetics; Wheat
• ISSN: 0016-6731; 1943-2631; 1943-2631
• DOI: 10.1534/genetics.166.1.461

The objectives of this study were to isolate and physically localize expressed resistance (R) genes on wheat chromosomes. Irrespective of the host or pest type, most of the 46 cloned R genes from 12 plant species share a strong sequence similarity, especially for protein domains and motifs. By utilizing this structural similarity to perform modified RNA fingerprinting and data mining, we identified 184 putative expressed R genes of wheat. These include 87 NB/LRR types, 16 receptor-like kinases, and 13 Pto-like kinases. The remaining were seven Hm1 and two Hs1(pro-1) homologs, 17 pathogenicity related, and 42 unique NB/kinases. About 76% of the expressed R-gene candidates were rare transcripts, including 42 novel sequences. Physical mapping of 121 candidate R-gene sequences using 339 deletion lines localized 310 loci to 26 chromosomal regions encompassing approximately 16% of the wheat genome. Five major R-gene clusters that spanned only approximately 3% of the wheat genome but contained approximately 47% of the candidate R genes were observed. Comparative mapping localized 91% (82 of 90) of the phenotypically characterized R genes to 18 regions where 118 of the R-gene sequences mapped.