Patient-derived small intestinal myofibroblasts direct perfused, physiologically responsive capillary development in a microfluidic Gut-on-a-Chip Model

• Published in: Scientific reports Vol. 10; no. 1; p. 3842
• Main Authors: Seiler, Kristen M., Bajinting, Adam, Alvarado, David M., Traore, Mahama A., Binkley, Michael M., Goo, William H., Lanik, Wyatt E., Ou, Jocelyn, Ismail, Usama, Iticovici, Micah, King, Cristi R., VanDussen, Kelli L., Swietlicki, Elzbieta A., Gazit, Vered, Guo, Jun, Luke, Cliff J., Stappenbeck, Thaddeus, Ciorba, Matthew A., George, Steven C., Meacham, J. Mark, Rubin, Deborah C., Good, Misty, Warner, Brad W.
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 02-03-2020; Nature Publishing Group
• Subjects: 13/106; 13/107; 13/51; 13/62; 14/19; 14/63; 631/1647/767/2199; 631/61/2035; Angiogenesis; Biomedical engineering; Capillaries - growth & development; Cell culture; Cell density; Coculture Techniques - instrumentation; Culture techniques; Digestive system; Drug therapy; Endothelial cells; Endothelium; Engineering; Epithelial cells; Fibroblasts; Gastroenterology; Gastrointestinal tract; Growth factors; Humanities and Social Sciences; Humans; Intestine, Small - cytology; Lab-On-A-Chip Devices; Medicine; Microfluidics; multidisciplinary; Myofibroblasts - cytology; Myofibroblasts - metabolism; Oxygen - metabolism; Oxygen tension; Pediatrics; Perfusion; Precision medicine; Science; Science (multidisciplinary); Small intestine; Stroma
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-020-60672-5

The development and physiologic role of small intestine (SI) vasculature is poorly studied. This is partly due to a lack of targetable, organ-specific markers for
in vivo
studies of two critical tissue components: endothelium and stroma. This challenge is exacerbated by limitations of traditional cell culture techniques, which fail to recapitulate mechanobiologic stimuli known to affect vessel development. Here, we construct and characterize a 3D
in vitro
microfluidic model that supports the growth of patient-derived intestinal subepithelial myofibroblasts (ISEMFs) and endothelial cells (ECs) into perfused capillary networks. We report how ISEMF and EC-derived vasculature responds to physiologic parameters such as oxygen tension, cell density, growth factors, and pharmacotherapy with an antineoplastic agent (Erlotinib). Finally, we demonstrate effects of ISEMF and EC co-culture on patient-derived human intestinal epithelial cells (HIECs), and incorporate perfused vasculature into a gut-on-a-chip (GOC) model that includes HIECs. Overall, we demonstrate that ISEMFs possess angiogenic properties as evidenced by their ability to reliably, reproducibly, and quantifiably facilitate development of perfused vasculature in a microfluidic system. We furthermore demonstrate the feasibility of including perfused vasculature, including ISEMFs, as critical components of a novel, patient-derived, GOC system with translational relevance as a platform for precision and personalized medicine research.