miR-30e-5p and miR-15a Synergistically Regulate Fatty Acid Metabolism in Goat Mammary Epithelial Cells via LRP6 and YAP1

• Published in: International journal of molecular sciences Vol. 17; no. 11; p. 1909
• Main Authors: Chen, Zhi, Qiu, Huiling, Ma, Liuan, Luo, Jun, Sun, Shuang, Kang, Kang, Gou, Deming, Loor, Juan J
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 16-11-2016; MDPI
• Subjects: Adaptor Proteins, Signal Transducing - genetics; Adaptor Proteins, Signal Transducing - metabolism; Animals; Epithelial Cells - metabolism; fat metabolism; Fatty acids; Fatty Acids - genetics; Fatty Acids - metabolism; Female; Gene expression; Gene Expression Regulation; Goats; Goats - genetics; Goats - metabolism; Humans; Lipoproteins; Low Density Lipoprotein Receptor-Related Protein-6 - genetics; Low Density Lipoprotein Receptor-Related Protein-6 - metabolism; LRP6; Mammary Glands, Animal - cytology; Mammary Glands, Animal - metabolism; Metabolism; MicroRNAs; MicroRNAs - genetics; MicroRNAs - metabolism; miR-15a; miR-30e-5p; Ruminantia; Up-Regulation; YAP1; YAP1; miR-15a; fat metabolism; miR-30e-5p; LRP6
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms17111909

MicroRNA (miRNA) regulates the expression of genes and influences a series of biological processes, including fatty acid metabolism. We screened the expression of miRNA in goat mammary glands during peak-lactation and non-lactating ("dry") periods, and performed an in vitro study with goat mammary epithelial cells (GMEC) prior to sequencing analysis. Results illustrated that miR-30e-5p and miR-15a were highly expressed. Utilizing a luciferase reporter assay and Western blot, low-density lipoprotein receptor-related protein 6 (
) and
(
) genes were demonstrated to be a target of miR-30e-5p and miR-15a in GMEC. Moreover, we demonstrated that the overexpression of miR-30e-5p and miR-15a in GMEC promoted fat metabolism while their knockdown impaired fat metabolism. These findings extend the discovery of a key role of miR-30e-5p and miR-15a in mediating adipocyte differentiation by suggesting a role in promoting milk fat synthesis. In conclusion, our findings indicate that miR-30e-5p, together with miR-15a, represses expression of
and promotes fat metabolism in GMEC. The data expanded our knowledge on the function of miRNAs in milk fat metabolism and synthesis in ruminant mammary cells.