Optimization of ionic liquid-based microwave-assisted extraction of polyphenolic content from Peperomia pellucida (L) kunth using response surface methodology

• Published in: Asian Pacific journal of tropical biomedicine Vol. 7; no. 7; pp. 660 - 665
• Main Authors: Ahmad, Islamudin, Yanuar, Arry, Mulia, Kamarza, Mun'im, Abdul
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01-07-2017; Department of Pharmaceutical Sciences, Faculty of Pharmacy, Universitas Indonesia, Depok 16424 West Java, Indonesia%Department of Pharmaceutical Sciences, Faculty of Pharmacy, Universitas Indonesia, Depok 16424 West Java, Indonesia%Department of Chemical Engineering, Faculty of Engineering, Universitas Indonesia, Depok 16424 West Java, Indonesia; Department of Pharmaceutical Sciences, Faculty of Pharmacy, Mulawarman University, Samarinda, East Kalimantan, Indonesia; Wolters Kluwer Medknow Publications
• Subjects: 1-buthyl-3-methylimidazolium; Box–Behnken design; Peperomia pellucida (L) Kunth; Polyphenolic content; Response surface methodology; Tetrafluoroborate; Tetrafluoroborate; Polyphenolic content; Box–Behnken design; Response surface methodology; 1-buthyl-3-methylimidazolium; Peperomia pellucida (L) Kunth
• ISSN: 2221-1691; 2588-9222
• DOI: 10.1016/j.apjtb.2017.06.010

Objective: To optimize the ionic liquid based microwave-assisted extraction (IL-MAE) of polyphenolic content from Peperomia pellucida (L) Kunth. Methods: The IL-MAE factors as experimental design parameters, including microwave power, extraction time, ionic liquid concentration, and liquid–solid ratio had been involved. Response surface methodology and Box–Behnken design were used to obtain predictive model (multivariate quadratic regression equation) and optimization of the extraction process. The response surface was analyzed by using the yields of total polyphenolic content as response value. Results: Based on the obtained results the optimum extraction condition, including microwave power of 30% Watts, extraction time of 18.5 min, the ionic liquid concen-tration of 0.79 mol/L, and the liquid–solid ratio of 10.72 mL/g 1-Buthyl-3-methylimidazolium tetrafluoroborate ([bmim]BF4) as a solvent was selected. The regression model was obtained to predicts the yields from Peperomia pellucida:Y = 30.250 – 1.356X1 + 2.655X2 + 2.252X3 – 0.565X4 + 0.990 X1X3 – 8.172 X1X4 – 3.439 X3X4 – 4.178 X12 – 3.210 X32 – 6.786 X42 – 7.290 X12X3 + 5.575 X1X32 – 4.843 X32X4 with R2 = 0.82519. Scale-up confirmation test was obtained the maximum yields of total polyphenolics content with the amount of 31.1725μg GAE/g. Conclusions: The IL-MAE method produced a higher extraction polyphenolic and performed rapidly, easily and efficiently.