Antiplatelet and antithrombotic activity of indole-3-carbinol in vitro and in vivo

• Published in: Phytotherapy research Vol. 22; no. 1; pp. 58 - 64
• Main Authors: Park, Min-Kyu, Rhee, Yun-Hee, Lee, Hyo-Jung, Lee, Eun-Ok, Kim, Kwan-Hyun, Park, Min-Jong, Jeon, Byung-Hun, Shim, Bum-Sang, Jung, Chang-Hyun, Choi, Seung-Hoon, Ahn, Kyoo-Seok, Kim, Sung-Hoon
• Format: Journal Article
• Language: English
• Published: Chichester, UK John Wiley & Sons, Ltd 01-01-2008; Wiley
• Subjects: Animals; antiplatelet activity; antithrombotic agent; Biological and medical sciences; Collagen - pharmacology; Dinoprostone - metabolism; Dose-Response Relationship, Drug; Epinephrine - metabolism; Fibrinogen - metabolism; Fibrinolytic Agents - chemistry; Fibrinolytic Agents - pharmacology; Flow Cytometry; General pharmacology; glycoprotein IIb/IIIa; Humans; indole-3-carbinol; Indoles - chemistry; Indoles - pharmacology; Medical sciences; Mice; Molecular Structure; Pharmacognosy. Homeopathy. Health food; Pharmacology. Drug treatments; Platelet Aggregation - drug effects; Platelet Aggregation Inhibitors - chemistry; Platelet Aggregation Inhibitors - pharmacology; Platelet Glycoprotein GPIIb-IIIa Complex - metabolism; Protein Binding - drug effects; thromboxane B2; Thromboxane B2 - metabolism; Glycoprotein IIbIIIa; Pharmacognosy; Arachidonic acid derivatives; Polyunsaturated fatty acid; Lipids; Thromboxane B2; glycoprotein IIb/IIIa; indole-3-carbinol; In vitro; antiplatelet activity; Antiplatelet agent; Protein; Medicinal plant; Eicosanoid; Plant origin; Antithrombotic agent; Unsaturated fatty acid; Indole derivatives
• ISSN: 0951-418X; 1099-1573
• DOI: 10.1002/ptr.2260

Indole-3-carbinol, a natural compound found in cruciferous vegetables, is known to have anticancer activity. In the present study, the antiplatelet and antithrombotic activities of indole-3-carbinol were investigated in vitro and in vivo. Indole-3-carbinol significantly inhibited collagen-induced platelet aggregation in human platelet rich plasma (PRP) in a concentration-dependent manner. Indole-3-carbinol significantly inhibited fibrinogen binding to the platelet surface glycoprotein IIb/IIIa (GP IIb/IIIa) receptor by flow cytometric analysis. In addition, the levels of thromboxane B₂ (TXB₂) and prostaglandin E₂ (PGE₂) in collagen stimulated PRP were significantly inhibited in a concentration-dependent manner by indole-3-carbinol. Furthermore, indole-3-carbinol dose-dependently suppressed the death of mice with pulmonary thrombosis induced by intravenous injection of collagen and epinephrine. These results suggest that indole-3-carbinol can be a potent antithrombotic agent with antiplatelet activity through the inhibition of GP IIb/IIIa receptor and thromboxane B₂ formation. Copyright © 2007 John Wiley & Sons, Ltd.