Intramuscular 17α-hydroxyprogesterone caproate administration attenuates immunoresponsiveness of maternal peripheral blood mononuclear cells

• Published in: American journal of obstetrics and gynecology Vol. 203; no. 6; pp. 561.e1 - 561.e5
• Main Authors: Foglia, Lisa M., MD, Ippolito, Danielle L., PhD, Stallings, Jonathan D., PhD, Zelig, Craig M., MD, Napolitano, Peter G., MD
• Format: Journal Article
• Language: English
• Published: New York, NY Mosby, Inc 01-12-2010; Elsevier
• Subjects: Biological and medical sciences; Cells, Cultured; Enzyme-Linked Immunosorbent Assay; Female; Gestational Age; Gynecology. Andrology. Obstetrics; Humans; Hydroxyprogesterones - administration & dosage; Immunomodulation; Immunosuppression - methods; In Vitro Techniques; inflammation; Injections, Intramuscular; interleukin-6; Interleukin-6 - immunology; Interleukin-6 - metabolism; Leukocytes, Mononuclear - drug effects; Leukocytes, Mononuclear - immunology; Medical sciences; Obstetrics and Gynecology; Pregnancy - blood; Pregnancy - immunology; Premature Birth - immunology; Premature Birth - prevention & control; progesterone; Reference Values; inflammation; interleukin-6; progesterone; Blood cell; Mononuclear cell; Mother; Hydroxyprogesterone caproate; Gynecology; Obstetrics; Intramuscular administration
• ISSN: 0002-9378; 1097-6868
• DOI: 10.1016/j.ajog.2010.07.016

Objective 17α-hydroxyprogesterone caproate (17P) may decrease risk of prematurity by suppressing maternal immunity. We hypothesized that in vivo 17P treatment attenuates immunoresponsiveness of peripheral blood mononuclear cells (PBMCs). Study Design Study subjects were gravidas receiving weekly prophylactic intramuscular 17P injections. Peripheral blood samples were obtained at 21-27 weeks' gestation. Gestational age–matched, drug-naïve gravidas served as controls. To simulate infection, isolated PBMCs were stimulated with lipoteichoic acid (LTA) or lipopolysaccharide (LPS). Extracellular interleukin-6 (IL-6) concentrations were quantified by an enzyme-linked immunosorbent assay. Results Unstimulated IL-6 levels were comparable in PBMCs derived from drug-naïve and 17P-treated subjects. LPS and LTA induced a dose-dependent elevation of IL-6 in control PBMCs. In patients who received exogenous 17P, LPS, and LTA stimulated induction of IL-6 was significantly decreased compared with controls ( P = .005 and P = .02). Conclusion In vivo 17P attenuated immunoreactivity of PBMCs in our in vitro model of Gram-positive and Gram-negative bacterial infection.