miR-198 functions as a tumor suppressor in breast cancer by targeting CUB domain-containing protein 1

• Published in: Oncology letters Vol. 13; no. 3; pp. 1753 - 1760
• Main Authors: Hu, Yingbin, Tang, Ziyuan, Jiang, Bonian, Chen, Juying, Fu, Zhongpin
• Format: Journal Article
• Language: English
• Published: Greece Spandidos Publications 01-03-2017; Spandidos Publications UK Ltd; D.A. Spandidos
• Subjects: Binding sites; Breast cancer; Care and treatment; Cell adhesion & migration; Cell growth; Classification; Development and progression; Genetic aspects; Health aspects; Kinases; Metastasis; MicroRNA; Oncology; Proteins; Tumor suppressor genes; Tumorigenesis; United States > US; China; cell migration; CUB domain-containing protein 1; breast cancer; cell proliferation; miR-198; cell adhesion
• ISSN: 1792-1074; 1792-1082
• DOI: 10.3892/ol.2017.5673

The molecular mechanisms underlying the dysregulation of microRNAs (miRs) have been previously documented in breast cancer. miR-198 has been reported to be deregulated in several human cancers. However, the detailed effects of miR-198 on breast cancer progression remain unclear. Using quantitative polymerase chain reaction analysis, we demonstrated in the present study that miR-198 was downregulated in breast cancer tissues and cell lines, and that downregulation of miR-198 was significantly correlated with lymph node metastasis. Functional studies revealed that miR-198 inhibited cell proliferation and migration and promoted cell adhesion in aggressive breast cancer cells
. In addition, we observed that CUB domain-containing protein 1 (CDCP1) was a direct target of miR-198, and that knockdown of CDCP1 inhibited cell proliferation and migration, and promoted cell adhesion, which was similar to the effects of overexpression of miR-198. Taken together, we provide evidence to characterize the role of miR-198/CDCP1 interaction in breast cancer, which may be useful in breast cancer therapy.