Sperm proteome of Mytilus galloprovincialis: Insights into the evolution of fertilization proteins in marine mussels

• Published in: Proteomics (Weinheim) Vol. 15; no. 23-24; pp. 4175 - 4179
• Main Authors: Zhang, Yanjie, Mu, Huawei, Lau, Stanley C. K., Zhang, Zhifeng, Qiu, Jian-Wen
• Format: Journal Article
• Language: English
• Published: Germany Blackwell Publishing Ltd 01-12-2015; Wiley Subscription Services, Inc
• Subjects: Animal proteomics; Animals; Evolution, Molecular; Male; Mussel; Mytilus - metabolism; Mytilus galloprovincialis; Proteins; Proteins - chemistry; Proteins - genetics; Proteins - metabolism; Proteome - metabolism; Sperm; Sperm protein; Spermatozoa - metabolism; Tandem Mass Spectrometry; Animal proteomics; Mussel; Mytilus galloprovincialis; Sperm; Sperm protein
• ISSN: 1615-9853; 1615-9861
• DOI: 10.1002/pmic.201500066

Cataloging the sperm proteome of an animal can improve our understanding of its sperm‐egg interaction and speciation, but such data are available for only a few free‐spawning invertebrates. This study aimed to identify the sperm proteome of Mytilus galloprovincialis, a free‐spawning marine mussel. We integrated public transcriptome datasets by de novo assembly, and applied SDS‐PAGE coupled LC‐MS/MS analysis to profile the sperm proteome, resulting in the identification of 550 proteins. Comparing the homologous sperm protein coding genes between M. galloprovincialis and its closely related species M. edulis revealed that fertilization proteins have the highest mean nonsynonymous substitution rate (Ka/Ks = 0.62) among 11 functional groups, consistent with previous reports of positive selection of several fertilization proteins in Mytilus. Moreover, 78 sperm proteins in different functional groups have Ka/Ks values > 0.5, indicating the presence of many candidate sperm proteins for further analysis of rapid interspecific divergence. The MS data are available in ProteomeXchange with the identifier PXD001665.