Comparative Analysis of Growth, Survival, and Virulence Characteristics of Listeria monocytogenes Isolated from Imported Meat

• Published in: Microorganisms (Basel) Vol. 12; no. 2; p. 345
• Main Authors: Pan, Xinye, Shen, Jinling, Hong, Yi, Wu, Yufan, Guo, Dehua, Zhao, Lina, Bu, Xiangfeng, Ben, Leijie, Wang, Xiang
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 07-02-2024; MDPI
• Subjects: Acid resistance; Bacteria; biological characteristics; Cell culture; Comparative analysis; Food; foodborne pathogen; Foodborne pathogens; Growth rate; Heat resistance; Infections; Lethality; Listeria; Listeria monocytogenes; Meat; Pathogenicity; Pathogens; pH effects; Physiology; potential pathogenicity; Reduction; Risk assessment; Serotypes; Survival; Thermal resistance; variability; Virulence; United States > US; China; foodborne pathogen; biological characteristics; virulence; variability; potential pathogenicity
• ISSN: 2076-2607; 2076-2607
• DOI: 10.3390/microorganisms12020345

is an important foodborne pathogen with worldwide prevalence. Understanding the variability in the potential pathogenicity among strains of different subtypes is crucial for risk assessment. In this study, the growth, survival, and virulence characteristics of 16
strains isolated from imported meat in China (2018-2020) were investigated. The maximum specific growth rate (
) and lag phase (
) were evaluated using the time-to-detection (TTD) method and the Baranyi model at different temperatures (25, 30, and 37 °C). Survival characteristics were determined by
-values and population reduction after exposure to heat (60, 62.5, and 65 °C) and acid (HCl, pH = 2.5, 3.5, and 4.5). The potential virulence was evaluated via adhesion and invasion to Caco-2 cells, motility, and lethality to
. The potential pathogenicity was compared among strains of different lineages and subtypes. The results indicate that the lineage I strains exhibited a higher growth rate than the lineage II strains at three growth temperatures, particularly serotype 4b within lineage I. At all temperatures tested, serotypes 1/2a and 1/2b consistently demonstrated higher heat resistance than the other subtypes. No significant differences in the log reduction were observed between the lineage I and lineage II strains at pH 2.5, 3.5, and 4.5. However, the serotype 1/2c strains exhibited significantly low acid resistance at pH 2.5. In terms of virulence, the lineage I strains outperformed the lineage II strains. The invasion rate to Caco-2 cells and lethality to
exhibited by the serotype 4b strains were higher than those observed in the other serotypes. This study provides meaningful insights into the growth, survival, and virulence of
, offering valuable information for understanding the correlation between the pathogenicity and subtypes of
.