Two distinct EF-Tu epitopes induce immune responses in rice and Arabidopsis

• Published in: Molecular plant-microbe interactions Vol. 27; no. 2; pp. 113 - 124
• Main Authors: Furukawa, Takehito, Inagaki, Hiroaki, Takai, Ryota, Hirai, Hiroyuki, Che, Fang-Sik
• Format: Journal Article
• Language: English
• Published: United States The American Phytopathological Society 01-02-2014
• Subjects: Acidovorax; Amino Acid Sequence; Arabidopsis; Arabidopsis - genetics; Arabidopsis - immunology; Arabidopsis - microbiology; Bacterial Proteins - genetics; Bacterial Proteins - metabolism; Bacterial Proteins - pharmacology; Brassicaceae; Cell Extracts - isolation & purification; Cell Extracts - pharmacology; Comamonadaceae - metabolism; Epitopes - immunology; Flagellin - genetics; Flagellin - metabolism; Gene Expression Profiling; Gene Expression Regulation, Plant; Glucans - metabolism; Hydrogen Peroxide - metabolism; Molecular Sequence Data; Oligonucleotide Array Sequence Analysis; Oryza - genetics; Oryza - immunology; Oryza - microbiology; Oryza sativa; Peptide Elongation Factor Tu - genetics; Peptide Elongation Factor Tu - metabolism; Peptide Elongation Factor Tu - pharmacology; Plant Diseases - immunology; Plant Diseases - microbiology; Plant Immunity; Plant Leaves - genetics; Plant Leaves - immunology; Plant Leaves - microbiology; Plant Proteins - genetics; Recombinant Proteins; Sequence Deletion
• ISSN: 0894-0282; 1943-7706
• DOI: 10.1094/mpmi-10-13-0304-r

Plants sense potential pathogens by recognizing conserved pathogen-associated molecular patterns (PAMPs) that cause PAMP-triggered immunity (PTI). We previously reported that rice recognizes flagellin from the rice-incompatible N1141 strain of Acidovorax avenae and subsequently induces immune responses. Cell extracts isolated from flagellin-deficient N1141 (Δfla1141) still induced PTI responses, suggesting that Δfla1141 possesses an additional PAMP distinct from flagellin. Here, we show that elongation factor Tu (EF-Tu), one of the most abundant bacterial proteins, acts as a PAMP in rice and causes several PTI responses. In Brassicaceae species, EF-Tu and an N-acetylated peptide comprising the first 18 amino acids of the N-terminus, termed elf18, are fully active as inducers of PTI responses. By contrast, elf18 did not cause any immune responses in rice, whereas an EF-Tu middle region comprising Lys176 to Gly225, termed EFa50, is fully active as a PAMP in rice. In the leaves of rice plants, EF-Tu induced H2O2 generation and callose deposition, and also triggered resistance to coinfection with pathogenic bacteria. Taken together, these data demonstrate that rice recognizes EFa50, which is distinct from elf18, and that this epitope induces PTI responses.