Strategies for optimizing heterologous protein expression in Escherichia coli

• Published in: Trends in biotechnology (Regular ed.) Vol. 16; no. 2; pp. 54 - 60
• Main Authors: Hannig, Gerhard, Makrides, Savvas C
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier Ltd 01-02-1998; Elsevier Science
• Subjects: Biological and medical sciences; Biotechnology; Codon; Cytoplasm - metabolism; Escherichia coli; Escherichia coli - genetics; expression systems; Fermentation; Fundamental and applied biological sciences. Psychology; Gene expression; Genetic engineering; Genetic technics; heterologous proteins; Methods. Procedures. Technologies; Modification of gene expression level; Molecular and cellular biology; Molecular genetics; Promoter Regions, Genetic; promoters; recombinant proteins; Recombinant Proteins - biosynthesis; heterologous proteins; recombinant proteins; promoters; Escherichia coli; expression systems; Heterologous system; Transcription promoter; Consensus sequence; Review; Gene expression; Optimization; Microorganism culture; Bacteria; Inclusion body; Recombinant protein; Hybrid gene; Enterobacteriaceae
• ISSN: 0167-7799; 1879-3096
• DOI: 10.1016/S0167-7799(97)01155-4

The many advantages of
Escherichia coli have ensured that it remains a valuable host for the efficient, cost-effective and high-level production of heterologous proteins. Here, we describe the current status of this prokaryotic expression system and focus on strategies designed to maximize the yields of recombinant proteins. Major challenges facing this expression system are also outlined.