Analytical approaches to uptake and release of hydrogel-associated FGF-2

• Published in: Journal of materials science. Materials in medicine Vol. 21; no. 3; pp. 915 - 923
• Main Authors: Zieris, A., Prokoph, S., Welzel, P. B., Grimmer, M., Levental, K. R., Panyanuwat, W., Freudenberg, U., Werner, C.
• Format: Journal Article Conference Proceeding
• Language: English
• Published: Boston Springer US 01-03-2010; Springer; Springer Nature B.V
• Subjects: Applied sciences; Binding sites; Biocompatible Materials - chemistry; Biological and medical sciences; Biomaterials; Biomedical Engineering and Bioengineering; Biomedical materials; Ceramics; Chemistry and Materials Science; Composites; Dose-Response Relationship, Drug; Enzyme-Linked Immunosorbent Assay - methods; Exact sciences and technology; Fibroblast Growth Factor 2 - chemistry; Fluorescent Dyes - chemistry; Glass; Heparin - chemistry; Humans; Hydrogels - chemistry; Iodine Radioisotopes - chemistry; Kinetics; Materials Science; Materials Testing; Medical sciences; Natural Materials; Organic polymers; Physicochemistry of polymers; Polyethylene Glycols - chemistry; Polymer Sciences; Properties and characterization; Protein Binding; Regenerative Medicine/Tissue Engineering; Solution and gel properties; Surfaces and Interfaces; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases; Technology. Biomaterials. Equipments; Thin Films; Time Factors; Water - chemistry; Immobilization Efficiency; Glass Cover Slip; Heparin; Amino Acid Analysis; Carboxylic Acid Group; Binding; Control release polymer; Analytical method; Exploration; Biomaterial; Basic fibroblast growth factor; Hydrogel; Comparative study; Biomedical engineering
• ISSN: 0957-4530; 1573-4838
• DOI: 10.1007/s10856-009-3913-z

Strategies to control the delivery of growth factors are critically important in the design of advanced biomaterials. In this study we investigated the binding and release of fibroblast growth factor 2 (FGF-2) to/from a biohybrid hydrogel matrix by four independent analytical methods: radioisotope and fluorescence labeling, amino acid analysis and Enzyme-Linked Immunosorbent Assays (ELISA). The compared analyses provided qualitatively similar uptake characteristics while the results of the FGF-2 quantification strongly depended on the particular experimental conditions. The release kinetics of FGF-2 from the gels could be monitored sensitively by
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I labeling and by ELISA-techniques. The latter method was concluded to be advantageous since it permits the application of unmodified (“native”) growth factors.