Phylogenetic diversity of isolates belonging to genera Geobacillus and Aeribacillus isolated from different geothermal regions of Turkey

• Published in: World journal of microbiology & biotechnology Vol. 27; no. 11; pp. 2683 - 2696
• Main Authors: Cihan, Arzu Coleri, Ozcan, Birgul, Tekin, Nilgun, Cokmus, Cumhur
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01-11-2011; Springer; Springer Nature B.V
• Subjects: Applied Microbiology; Bacteria; Biochemistry; Biological and medical sciences; Biomedical and Life Sciences; Biotechnology; Clusters; Environmental Engineering/Biotechnology; Fundamental and applied biological sciences. Psychology; Genes; Geothermal; Life Sciences; Microbiology; Morphology; Motility; Original Paper; Phylogenetics; Ribosomal DNA; Similarity; Strain; Taxonomy; Temperature; Turkey; ARDRA; Isolation; Thermophilic; 16S rRNA gene; Diversity; Thermophily; Phylogeny; Aeribacillus; Gene; Ribosomal RNA; 16S-RNA; Isolate; Geobacillus
• ISSN: 0959-3993; 1573-0972
• DOI: 10.1007/s11274-011-0742-2

The phylogenetic diversity of 31 thermophilic bacilli belonging to genera
Geobacillus
and
Aeribacillus
were investigated which were isolated from various geothermal sites of Turkey. Twenty-seven of these isolates were found to be belonged within the genus
Geobacillus
, whereas 4 of them were identified as
Aeribacillus pallidus
. The comparative 16S rRNA gene sequence analyses revealed that the
A. pallidus
isolates displayed sequence similarity values from 98.0 to 99.6% to their closest relative. Furthermore,
Geobacillus
isolates showed sequence similarity values from 88.9 to 99.8% with the reference type strains. According to the phylogenetic analysis, isolates belonging to genus
Geobacillus
were diverged into nine clusters and among these isolates, 19 of them were identified as strains related to
G. caldoproteolyticus
,
G. thermodenitrificans
,
G. stearothermophilus
,
G. thermoglucosidasius
and
G. toebii
with the most abundant 13 isolates from
G.
caldoproteolyticus
. Four of the
Geobacillus
isolates were named as unidentified mix group, as they found to be genetically very homogenous like their closely related type species:
G. thermoleovorans
,
G. vulcani
,
G. lituanicus
,
G. kaustophilus
,
G. caldovelox
,
G. caldotenax
, and
G. uralicus
. Moreover, the sequence comparisons of E173a, E265, C161ab and A142 isolates demonstrated that they represented novel species among genus
Geobacillus
as they shared lower than 96.7% sequence similarity to all the described type species. The
Alu
I-,
Hae
III- and
Taq
I-ARDRA results were in congruence with the 16S rRNA gene sequence analyses. By ARDRA results, the isolates were able to be differentiated and clustered, the discriminative restriction fragments of these isolates and type species were determined and the novelty of E173, E265, C161ab and A142 isolates could be displayed. Some differentiating phenotypic characters and the ability of amylase, glucosidase and protease production of these bacilli were also studied and biotechnologically valuable thermostable enzyme producing isolates were introduced in order to use in further studies.